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Titolo:
Auxin and cytokinin have opposite effects on amyloplast development and the expression of starch synthesis genes in cultured Bright Yellow-2 tobacco cells
Autore:
Miyazawa, Y; Sakai, A; Miyagishima, S; Takano, H; Kawano, S; Kuroiwa, T;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sci, Dept Biol Sci, Tokyo 1130033, Japan
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 2, volume: 121, anno: 1999,
pagine: 461 - 469
SICI:
0032-0889(199910)121:2<461:AACHOE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE PYROPHOSPHORYLASE; BY-2 CELLS; BIOSYNTHESIS; PLANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Miyazawa, Y Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 iol Sci, Tokyo 1130033, Japan
Citazione:
Y. Miyazawa et al., "Auxin and cytokinin have opposite effects on amyloplast development and the expression of starch synthesis genes in cultured Bright Yellow-2 tobacco cells", PLANT PHYSL, 121(2), 1999, pp. 461-469

Abstract

In cultured Bright Yellow-2 (BY-2) tobacco (Nicotiana tabacum) cells, the depletion of auxin (2,4-dichlorophenoxyacetic acid) in the culture medium induces the accumulation of starch. This is accelerated by the addition of cytokinin (benzyladenine). Light and electron microscopic observations revealed that this amyloplast formation involves drastic changes in plastid morphology. The effects of auxin and cytokinin on amyloplast development were investigated by adding auxin or cytokinin to cells grown in a hormone-free culture. Auxin repressed amyloplast development, whereas cytokinin accelerated starch accumulation regardless of the timing of hormone addition. RNA gel-blot analysis revealed that the accumulation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS), granule-bound starch synthase, and starch branching enzyme transcripts were also affected by hormonal conditions. High levels of AgpS, granule-bound starch synthase, and starch branching enzyme transcripts accumulated in amyloplast-developing cells grown in auxin-depleted conditions. Furthermore, the addition of auxin to the cells cultured in hormone-free medium reduced the level of AgpS transcripts, whereas the addition of cytokinin increased it, irrespective of the timing of hormoneaddition. These results suggest that auxin and cytokinin exert opposite effects on amyloplast development by regulating the expression of the genes required for starch biosynthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:56:54