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Titolo:
Mu and kappa(1) opioid-stimulated [S-35]guanylyl-5 '-O-(gamma-thio)-triphosphate binding in cynomolgus monkey brain
Autore:
Sim-Selley, LJ; Daunais, JB; Porrino, LJ; Childers, SR;
Indirizzi:
Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Med Coll Virginia, Inst Alcohol & Drug Studies, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Wake Forest Univ, Sch Med, Dept Physiol & Pharmacol, Ctr Invest Neurosci, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA Wake Forest Univ, Sch Med, Ctr Neurobiol Invest Drug Abuse, Winston Salem,NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 e, Winston Salem,NC 27157 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 94, anno: 1999,
pagine: 651 - 662
SICI:
0306-4522(1999)94:2<651:MAKO['>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG BRAIN; GAMMA-S AUTORADIOGRAPHY; RECEPTOR-LIGAND BINDING; ACTIVATED G-PROTEINS; RAT-BRAIN; SIGNAL-TRANSDUCTION; NUCLEUS-ACCUMBENS; H-3 BREMAZOCINE; HUMAN FOREBRAIN; HUMAN STRIATUM;
Keywords:
[S-35]GTP gamma S autoradiography; mu opioid receptor; kappa(1) opioid receptor; G-protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Sim-Selley, LJ Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 23298 USA
Citazione:
L.J. Sim-Selley et al., "Mu and kappa(1) opioid-stimulated [S-35]guanylyl-5 '-O-(gamma-thio)-triphosphate binding in cynomolgus monkey brain", NEUROSCIENC, 94(2), 1999, pp. 651-662

Abstract

Agonist-stimulated [S-35]GTP gamma S binding allows the visualization of receptor-activated G-proteins, thus revealing the anatomical localization offunctional receptor activity. Ln the present study, agonist-stimulated [S-35]GTP gamma S binding was used to demonstrate mu and kappa(1) opioid-stimulated [S-35]GTP gamma S binding in tissue sections and membranes from cynomolgus monkey brain using DAMGO and U50,488H, respectively. Concentrations of agonists required to produce maximal stimulation of [S-35]GTP gamma S binding were determined in membranes from the frontal poles of the brain. Receptor specificity was verified in both membranes and sections by inhibiting agonist-stimulated [S-35]GTP gamma S binding with the appropriate antagonist. Mu opioid-stimulated [S-35]GTP gamma S binding was high in areas including the amygdala, ventral striatum, caudate, putamen, medial thalamus and hypothalamus. Dense mu-stimulated [S-35]GTP gamma S binding was also found inbrainstem nuclei including the interpeduncular nucleus, parabrachial nucleus and nucleus of the solitary tract. Kappa(1) opioid-stimulated [S-35]GTP gamma S binding was high in limbic and association cortex, ventral striatum, caudate, putamen, globus pallidus, claustrum, amygdala, hypothalamus and substantia nigra. These results demonstrate the applicability of [S-35]GTP gamma S autoradiography to examine receptor-activated G-proteins in the primate brain and reveal functional mu and kappa(1) opioid receptor activity that may contribute to the reported central nervous system effects of opiates. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 16:06:55