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Titolo:
Electroenzyme-catalyzed oxidation of styrene and cis-beta-methylstyrene using thin films of cytochrome P450cam and myoglobin
Autore:
Zu, XL; Lu, ZQ; Zhang, Z; Schenkman, JB; Rusling, JF;
Indirizzi:
Univ Connecticut, Dept Chem, Storrs, CT 06269 USA Univ Connecticut StorrsCT USA 06269 cut, Dept Chem, Storrs, CT 06269 USA Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA Univ Connecticut Farmington CT USA 06032 rmacol, Farmington, CT 06032 USA
Titolo Testata:
LANGMUIR
fascicolo: 21, volume: 15, anno: 1999,
pagine: 7372 - 7377
SICI:
0743-7463(19991012)15:21<7372:EOOSAC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTENTIAL PROTECTIVE MECHANISM; BIOMEMBRANE-LIKE FILMS; ABSOLUTE STEREOCHEMISTRY; SUBSTRATE HYDROXYLATION; HYDROGEN-PEROXIDE; FERRYL-MYOGLOBIN; EPOXIDATION; P450(CAM); IDENTIFICATION; HEMOGLOBIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Rusling, JF Univ Connecticut, Dept Chem, U-60, Storrs, CT 06269 USA Univ Connecticut U-60 Storrs CT USA 06269 Storrs, CT 06269 USA
Citazione:
X.L. Zu et al., "Electroenzyme-catalyzed oxidation of styrene and cis-beta-methylstyrene using thin films of cytochrome P450cam and myoglobin", LANGMUIR, 15(21), 1999, pp. 7372-7377

Abstract

Protein-polyion films grown layer-by-layer and cast protein-surfactant films were employed on electrodes for catalytic oxidation of styrene derivatives to epoxides. Cytochrome P450cam and myoglobin in these films mediated the electrochemical reduction of oxygen to hydrogen peroxide, which activatesthese heme proteins to catalyze olefin oxidation. Compared to bare electrodes with the proteins dissolved in solution, ultrathin protein-polyion films on Au electrodes coated with mercaptopropane sulfonate gave the best catalytic activities for the oxidations. Improved performance of protein-polyion films is related to efficient, reversible heme Fe-III/Fe-II electron transfer and better mechanical stability than the surfactant films. Furthermore, dependence of product stereochemistry on oxygen availability in the reaction medium for the oxidation of cis-beta-methylstyrene suggested two pathways for olefin oxidation, which had not been reported previously for cyt P450 enzymes. The stereoselective pathway depends on an active, high-valent iron-oxygen intermediate as in the natural enzyme system, while the nonstereoselective pathway may involve a peroxyl radical near the protein surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:57:57