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Titolo:
Circuit-specific coinfection of neurons in the rat central nervous system with two pseudorabies virus recombinants
Autore:
Kim, JS; Enquist, LW; Card, JP;
Indirizzi:
Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 eurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 sychiat, Pittsburgh, PA 15260 USA Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA Taegu Univ, Dept Phys Therapy, Taegu, South Korea Taegu Univ Taegu SouthKorea niv, Dept Phys Therapy, Taegu, South Korea
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 11, volume: 73, anno: 1999,
pagine: 9521 - 9531
SICI:
0022-538X(199911)73:11<9521:CCONIT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERPES-SIMPLEX VIRUS; VISUAL-SYSTEM; TRANSNEURONAL TRANSPORT; GLYCOPROTEIN-D; BETA-GALACTOSIDASE; INFECTION; TYPE-1; GI; NEUROTROPISM; ANTEROGRADE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Card, JP Univ Pittsburgh, Dept Neurosci, 446 Crawford Hall, Pittsburgh, PA15260 USA Univ Pittsburgh 446 Crawford Hall Pittsburgh PA USA 15260 260 USA
Citazione:
J.S. Kim et al., "Circuit-specific coinfection of neurons in the rat central nervous system with two pseudorabies virus recombinants", J VIROLOGY, 73(11), 1999, pp. 9521-9531

Abstract

Neurotropic alphaherpesviruses have become popular tools for transynaptic analysis of neural circuitry. It has also been demonstrated that coinfection with two viruses expressing unique reporters can be used to define more complicated circuitry. However, the coinfection studies reported to date have employed nonisogenic strains that differ in their invasive properties. Inthe present investigation we used two antigenically distinct recombinants of the swine pathogen pseudorabies virus (PRV) in single and double infections of the rat central nervous system. Both viruses are derivatives of PRV-Bartha, a strain with reduced virulence that is widely used for circuit analysis. PRV-BaBlu expresses beta-galactosidase, and PRV-D expresses the PRV membrane protein gI, the gene for which is deleted in PRV-BaBlu, Antibodiesto beta-galactosidase identify neurons infected with PRV-BaBlu, and antibodies monospecific for PRV gI identify neurons infected with PRV-D, The ability of these strains to establish coinfections in neurons was evaluated in visual and autonomic circuitry in which the parental virus has previously been characterized. The following conclusions can be drawn from these experiments. First, PRV-D is significantly more neuroinvasive than PRV-Bartha or PRV-BaBlu in the same circuitry. Second, PRV-D is more virulent than eitherPRV-Bartha or PRV-BaBlu, and PRV-BaBlu is less virulent than PRV-Bartha. Third, in every model examined, PRV-D and PRV-BaBlu coinfect some neurons, but single infections predominate. Fourth, prior infection with one virus renders neurons less permissive to infection by another virus. Fifth, prior infection by PRV-D is more effective than PRV-BaBlu in reducing invasion andspread of the second virus. Collectively, the data define important variables that must be considered in coinfection experiments and suggest that themost successful application of this approach would be accomplished by using isogenic strains of virus with equivalent virulence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:49:23