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Titolo:
Analysis of HIV-1 in the cervicovaginal secretions and blood of pregnant and nonpregnant women
Autore:
Shaheen, F; Sison, AV; McIntosh, L; Mukhtar, M; Pomerantz, RJ;
Indirizzi:
Thomas Jefferson Univ, Jefferson Med Coll, Div Infect Dis,Dept Med, Dorrance H Hamilton Labs,Ctr Human Virol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Med Coll Penn & Hahnemann Univ, Dept Obstet & Gynecol, Philadelphia, PA USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA Philadelphia, PA USA Robert Wood Johnson Univ Hosp, Hamilton, NJ USA Robert Wood Johnson Univ Hosp Hamilton NJ USA niv Hosp, Hamilton, NJ USA
Titolo Testata:
JOURNAL OF HUMAN VIROLOGY
fascicolo: 3, volume: 2, anno: 1999,
pagine: 154 - 166
SICI:
1090-9508(199905/06)2:3<154:AOHITC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; FEMALE GENITAL-TRACT; TYPE-1 RNA; REVERSE TRANSCRIPTION; SEXUAL TRANSMISSION; CERVICAL SECRETIONS; MACROPHAGE TROPISM; VIRAL PHENOTYPE; INFECTED WOMEN; CELL TROPISM;
Keywords:
HIV-1; transmission; genotype; cervix; cervicovaginal secretions; pregnancy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Pomerantz, RJ Thomas Jefferson Univ, Jefferson Med Coll, Div Infect Dis,Dept Med, Dorrance H Hamilton Labs,Ctr Human Virol, Jefferson Alumni Hall,1020 Locust St,Suite 329, Philadelphia, PA 19107 USA Thomas Jefferson Univ Jefferson Alumni Hall,1020 Locust St,Suite 329 Philadelphia PA USA 19107
Citazione:
F. Shaheen et al., "Analysis of HIV-1 in the cervicovaginal secretions and blood of pregnant and nonpregnant women", J HUMAN VIR, 2(3), 1999, pp. 154-166

Abstract

Objectives: To detect HIV-1 in cellular and acellular fractions of cervicovaginal secretions obtained by cervicovaginal lavage (CVL) and evaluate viral genotypes in the HIV-1-positive CVL samples. Study Design/Methods: This study consists of 37 HIV-1-seropositive pregnant and nonpregnant women from the United States. A total of 63 paired CVL and blood samples were collected. HIV-1 DNA from cervical cells (CC) and virion RNA from cervical supernatant (CS) was detected by gag polymerase chain reaction (PCR) assays. The HIV-1 genotypes were determined by analyzing thenested PCR-amplified V3 region sequences of the HIV-1 gp120 envelope gene. Results: Within this cohort, 95% of the women were on single or combination antiretroviral therapy. Of the pregnant women, 63% of samples had HIV-1 viral DNA in the CC, and 29% of samples were positive for viral RNA in the CS. Among nonpregnant women, 71% of samples were positive for HIV-1 DNA in CC, and 46% of samples tested positive for virion RNA in CS. Plasma viral load ranged between 10,000 and 100,000 copies/mL and showed significant correlation with the detection of HIV-1 RNA in the CVL; this relation was less apparent with viral DNA in CC. The viral blood and CVL specimens were further analyzed by evaluating the genotypes of HIV-1 variants. In most patients,a high degree of similarity was observed between the viral sequences derived from blood and CVL samples. Two patients demonstrated closely related but somewhat distinct genotypic variants in CVL and blood. One subject showedclear compartmentalization in which distinct viral genotypes were observedin CVL and blood. Based on V3 loop analyses of gp120, with one exception, the cervicovaginal secretions harbored viral populations with a macrophage (CCR5)-tropic phenotype. Conclusions: This study demonstrates the unique characteristics of HIV-1 strains in the genital secretions of a relatively large cohort of HIV-1-infected women in the United States. These results are important for further analysis of HIV-1 transmission and pathogenesis in vivo and for rational vaccine design.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:54:22