Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification and characterization of Drosophila homolog of Rho-kinase
Autore:
Mizuno, T; Amano, M; Kaibuchi, K; Nishida, Y;
Indirizzi:
Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan Nagoya Univ Nagoya Aichi Japan 4648602 a Ku, Nagoya, Aichi 4648602, Japan Nara Inst Sci & Technol, Div Signal Transduct, Ikoma 6300101, Japan Nara Inst Sci & Technol Ikoma Japan 6300101 nsduct, Ikoma 6300101, Japan
Titolo Testata:
GENE
fascicolo: 2, volume: 238, anno: 1999,
pagine: 437 - 444
SICI:
0378-1119(19991001)238:2<437:IACODH>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEIN; CELL-SHAPE CHANGES; MYOTONIC-DYSTROPHY KINASE; SERINE THREONINE KINASE; ACTIN STRESS FIBERS; SERINE/THREONINE KINASE; MYOSIN PHOSPHATASE; ROK-ALPHA; CDC42; FAMILY;
Keywords:
actin cytoskeleton; in-vitro kinase assay; low-stringency PCR; morphogenesis; Rho effector; two-hybrid analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Nishida, Y Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan Nagoya Univ Nagoya Aichi Japan 4648602 a, Aichi 4648602, Japan
Citazione:
T. Mizuno et al., "Identification and characterization of Drosophila homolog of Rho-kinase", GENE, 238(2), 1999, pp. 437-444

Abstract

The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho-kinase/ROK alpha, one of the targets of Rho, has been shown tobind to Rho in GTP-bound form and to phosphorylate the myosin light chain (MLC) and the myosin-binding subunit (MBS) of myosin phosphatase, resultingin the activation of myosin. Thus, Rho-kinase/ROK alpha has been suggestedto play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho-kinase/ROK alpha, DRho-kinase, which has conserved the basic structural feature of Rho-kinase/ROK alpha consisting of the N-terminal kinase, central coiled-coil and C-terminal pleckstrin homology (PH) domains. A two-hybrid analysis demonstrated that DRho-kinase interacts with the GTP-bound form of the Drosophila Rho, Drhol, at the conserved Rho-binding site. DRho-kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho-kinase, in vitro. DRho-kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drhol. These results suggest that DRho-kinase is an effector of Drhol. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:36:51