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Titolo:
Routine identification of Campylobacter jejuni and Campylobacter coli fromhuman stool samples
Autore:
Steinbrueckner, B; Haerter, G; Pelz, K; Kist, M;
Indirizzi:
Univ Freiburg, Inst Med Mikrobiol & Hyg, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 & Hyg, D-79104 Freiburg, Germany
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 179, anno: 1999,
pagine: 227 - 232
SICI:
0378-1097(19991015)179:2<227:RIOCJA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; FATTY-ACID COMPOSITION; HELICOBACTER-PULLORUM; GENE; DISCRIMINATION; AMPLIFICATION; ORGANISMS;
Keywords:
Campylobacter jejuni; Campylobacter coli; gas chromatography; biochemical identification; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Steinbrueckner, B Klinikum Ingolstadt, Inst Lab Med, Krumenauerstr 25, D-85049 Ingolstadt, Germany Klinikum Ingolstadt Krumenauerstr 25 Ingolstadt Germany D-85049
Citazione:
B. Steinbrueckner et al., "Routine identification of Campylobacter jejuni and Campylobacter coli fromhuman stool samples", FEMS MICROB, 179(2), 1999, pp. 227-232

Abstract

Correct identification of Campylobacter jejuni and Campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. The widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. This investigation was performed to assess the performance of common biochemical identification in comparison with species-specific PCR and gas liquid chromatography of whole cellfatty acid extracts (GLC). A total of 150 consecutive isolates from human stool samples were investigated (134 C. jejuni ssp. jejuni, 14 C. coil, twoHelicobacter pullorum). From these 144, 145 and 149 isolates were correctly identified by biochemistry, GLC and PCR, respectively. Biochemical identification of all C. jejuni isolates was confirmed by PCR. GLC detected both H. pullorum strains but misidentified two C. coli strains as C. jejuni and one C. jejuni strain as C. coil, No single method can be defined as 'gold standard' for identification of C. jejuni and C. coli but a combination of techniques is needed. Therefore a stepwise identification scheme starting with biochemical reactions is suggested. All results other than C. jejuni should be confirmed by further methods. For indoxyl acetate-positive isolates species-specific PCR is recommended while GLC seems to be advantageous in indoxyl acetate-negative isolates. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:13:53