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Titolo:
Kinetics of 3-[I-123]iodo-L-alpha-methyltyrosine transport in rat C6 glioma cells
Autore:
Riemann, B; Stogbauer, F; Kopka, K; Halfter, H; Lasic, M; Schirmacher, A; Kuwert, T; Weckesser, M; Ringelstein, EB; Schober, O;
Indirizzi:
Univ Munster, Dept Nucl Med, D-48129 Munster, Germany Univ Munster Munster Germany D-48129 Nucl Med, D-48129 Munster, Germany Univ Munster, Dept Neurol, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 Dept Neurol, D-4400 Munster, Germany Univ Erlangen Nurnberg, Dept Nucl Med, D-8520 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-8520 D-8520 Erlangen, Germany
Titolo Testata:
EUROPEAN JOURNAL OF NUCLEAR MEDICINE
fascicolo: 10, volume: 26, anno: 1999,
pagine: 1274 - 1278
SICI:
0340-6997(199910)26:10<1274:KO3TIR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; ALPHA-METHYL TYROSINE; BLOOD-BRAIN-BARRIER; L-3-IODO-ALPHA-METHYL TYROSINE; IODINE-123-ALPHA-METHYL TYROSINE; CEREBRAL GLIOMAS; TUMOR UPTAKE; SPECT; ASTROCYTES; PET;
Keywords:
rat C6 glioma cells; L-[I-123]iodo-alpha-methyltyrosine; L-system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Riemann, B Univ Munster, Dept Nucl Med, Albert Schweitzer Str 33, D-48129 Munster, Germany Univ Munster Albert Schweitzer Str 33 Munster Germany D-48129
Citazione:
B. Riemann et al., "Kinetics of 3-[I-123]iodo-L-alpha-methyltyrosine transport in rat C6 glioma cells", EUR J NUCL, 26(10), 1999, pp. 1274-1278

Abstract

3-[I-123]Iodo-L-alpha-methyltyrosine (I-123-IMT) is used for the diagnosisand monitoring of brain rumours by means of single-photon emission tomography (SPET). To date, little has been known about the system for the transport of I-123-IMT into brain tumour cells. It is assumed that I-123-IMT is transported by a specific carrier for large, neutral amino acids (L-system). In this study, rat C6 glioma cells were used to characterize the uptake system of I-123-IMT and to investigate its precise kinetics. The time course of I-123-IMT uptake into the cells was examined for a range of 1-60 min. I-123-IMT uptake rates with varying concentrations of I-123-IMT (2.5-50 mu M) in the medium were quantified to assess the kinetic parameters of I-123-IMTtransport. Furthermore, competition of I-123-IMT with other amino acids was investigated to identify the distinct transport systems involved in I-123-IMT uptake. I-123-IMT uptake into C6 glioma cells was linear for approximately 10 min and reached a steady-state level within 30 min. The analysis ofthe rate of uptake of I-123-IMT at different concentrations was concordantwith the predominance of a single uptake system. The apparent Michaelis constant (K-m) of I-123-IMT was 26.2+/-1.9 mu M, and the maximum transport velocity (V-max) was 35.4+/-1.7 nmol/mg protein per 10 min. 77%+/-10% of I-123-IMT transport was sodium independent and 23%+/-3% was sodium dependent. Competitive inhibition of I-123-IMT uptake by 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid, alpha-(methylamino)isobutyric acid and naturally occurring amino acids revealed a major I-123-IMT transport via the sodium-independent system L (72%) and a minor uptake via the sodium-dependent system B-0,B- (17%). Our results show that I-123-IMT trans port into C6 glioma cells isprincipally mediated by the L-system and to a minor extent by the B-0,B-+-system. The kinetic parameters of I-123-IMT uptake are in the range of those of naturally occurring amino acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 11:50:22