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Titolo:
Expansion of circulating V gamma 9/V delta 1 T cells in a patient with a syndrome of recurrent fever: evidence for an unusual antigen-driven process leading to selection of recurrent motifs within TCR junctional loops of diverse lengths
Autore:
Jouen-Beades, F; Halary, F; Drouot, L; Peyrat, MA; Paris, E; Joly, P; Gilbert, D; Bonneville, M; Tron, F;
Indirizzi:
Hop Charles Nicolle, Fac Mixte Med & Pharm, Inst Federat Rech Multidisciplinaire Peptides IFR, INSERM,U519, F-76031 Rouen, France Hop Charles Nicolle Rouen France F-76031 ERM,U519, F-76031 Rouen, France Inst Biol, INSERM, U463, Nantes, France Inst Biol Nantes FranceInst Biol, INSERM, U463, Nantes, France
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 10, volume: 29, anno: 1999,
pagine: 3338 - 3349
SICI:
0014-2980(199910)29:10<3338:EOCVG9>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
KILLER INHIBITORY RECEPTORS; GAMMA-DELTA REPERTOIRE; PERIPHERAL-BLOOD; MULTIPLE-SCLEROSIS; HIV-INFECTION; ACTIVATION MARKERS; LYMPHOCYTES-T; IN-VIVO; RECOGNITION; EXPRESSION;
Keywords:
gamma delta T cell; v delta 1 repertoire; v delta 9 repertoire; recurrent fever;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Tron, F Fac Mixte Med & Pharm, INSERM, U519, 22 Blvd Gambetta, F-76183 Rouen, France Fac Mixte Med & Pharm 22 Blvd Gambetta Rouen France F-76183 rance
Citazione:
F. Jouen-Beades et al., "Expansion of circulating V gamma 9/V delta 1 T cells in a patient with a syndrome of recurrent fever: evidence for an unusual antigen-driven process leading to selection of recurrent motifs within TCR junctional loops of diverse lengths", EUR J IMMUN, 29(10), 1999, pp. 3338-3349

Abstract

Polyclonal expansions of human V delta 1 T cells have been described in diverse physiopathological situations without strong TCR structural data for an antigen-driven selection. Here, we have analyzed the phenotype and TCR repertoire of gamma delta T cells obtained from the peripheral blood of a 19-year-old patient with a syndrome of recurrent fever, which accounted for up to 40 % of CD3(+) T cells and expressed predominantly V gamma 9 and V delta 1 TCR regions and a memory phenotype. Sequence analysis of V delta 1-J delta 1 transcripts derived from peripheral blood lymphocytes (PBL) indicated that, while V delta 1-J delta 1 junctional sequences were diverse in length, all but one contained several recurrent motifs at conserved positions from both the 5'- and 3'-ends of the complementarity-determining region (CDR)3 loop. Analysis of gamma delta T cell clones derived from patient PBL demonstrated that V gamma 9(+) but not V gamma 9(-) T cell clones frequently expressed V delta 1 chains with these characteristics and unveiled a hierarchy between the constraints imposed on the 5'- vs. the 3' motifs of the V delta 1 CDR3 loops. These results constitute the first strong evidence for a nominal antigen-driven selection of V delta 1 T cells in vivo and also suggest that the hierarchy of the constraints imposed by antigens respectively on the length and amino acid composition of TCR CDR3 loops differs between alpha beta and gamma delta T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 03:59:14