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Titolo:
A VACUOLAR-TYPE H-ATPASE IN A NONVACUOLAR ORGANELLE IS REQUIRED FOR THE SORTING OF SOLUBLE VACUOLAR PROTEIN PRECURSORS IN TOBACCO CELLS()
Autore:
MATSUOKA K; HIGUCHI T; MAESHIMA M; NAKAMURA K;
Indirizzi:
UNIV CALIF BERKELEY,DEPT MOL & CELL BIOL,401 BARKER HALL BERKELEY CA 94720 NAGOYA UNIV,SCH AGR SCI,BIOCHEM LAB,CHIKUSA KU NAGOYA AICHI 46401 JAPAN
Titolo Testata:
The Plant cell
fascicolo: 4, volume: 9, anno: 1997,
pagine: 533 - 546
SICI:
1040-4651(1997)9:4<533:AVHIAN>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT-TIP CELLS; PLANT-CELLS; TRANSLOCATING ATPASE; CORN COLEOPTILES; BARLEY LECTIN; MAIZE ROOTS; MEMBRANE; TONOPLAST; GOLGI; PYROPHOSPHATASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
K. Matsuoka et al., "A VACUOLAR-TYPE H-ATPASE IN A NONVACUOLAR ORGANELLE IS REQUIRED FOR THE SORTING OF SOLUBLE VACUOLAR PROTEIN PRECURSORS IN TOBACCO CELLS()", The Plant cell, 9(4), 1997, pp. 533-546

Abstract

In plant cells, vacuolar matrix proteins are separated from the secretory proteins at the Golgi complex for transport to the vacuoles. To investigate the involvement of vacuolar-type ATPase (V-ATPase) in the vacuolar targeting of soluble proteins, we analyzed the effects of bafilomycin A(1) and concanamycin A on the transport of vacuolar protein precursors in tobacco cells. Low concentrations of these inhibitors caused the missorting of several vacuolar protein precursors; sorting wasmore sensitive to concanamycin A than to bafilomycin A(1). Secretion of soluble proteins from tobacco cells was also inhibited by bafilomycin A(1) and concanamycin A. We next analyzed the subcellular localization of V-ATPase. V-ATPase was found in a wide variety of endomembrane organelles. Both ATPase activity and ATP-dependent proton-pumping activity in the Golgi-enriched fraction were more sensitive to concanamycin A than to bafilomycin A(1), whereas these activities in the tonoplast fraction were almost equally sensitive to both reagents. Our observations indicate that the V-ATPase in the organelle that was recovered in the Golgi-enriched fraction is required for the transport of vacuolar protein precursors and that this V-ATPase is distinguishable from the tonoplast-associated V-ATPase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:39:18