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Titolo:
Reduced antilisterial activity of TNF-deficient bone marrow-derived macrophages is due to impaired superoxide production
Autore:
Muller, M; Althaus, R; Frohlich, D; Frei, K; Eugster, HP;
Indirizzi:
Univ Zurich Hosp, Dept Internal Med, Clin Immunol Sect, CH-8044 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8044 CH-8044 Zurich, Switzerland Novartis Pharma Res, Basel, Switzerland Novartis Pharma Res Basel Switzerland is Pharma Res, Basel, Switzerland Biol Res Inst, Matzingen, Switzerland Biol Res Inst Matzingen Switzerland ol Res Inst, Matzingen, Switzerland Univ Zurich Hosp, Dept Neurosurg, Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland Dept Neurosurg, Zurich, Switzerland Univ Hosp Regensburg, Dept Anaesthesia, Regensburg, Germany Univ Hosp Regensburg Regensburg Germany naesthesia, Regensburg, Germany
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 10, volume: 29, anno: 1999,
pagine: 3089 - 3097
SICI:
0014-2980(199910)29:10<3089:RAAOTB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; CELL-INDEPENDENT PATHWAY; NITRIC-OXIDE SYNTHASE; LISTERIA-MONOCYTOGENES; IFN-GAMMA; MURINE LISTERIOSIS; ANTIBACTERIAL RESISTANCE; INTERFERON-GAMMA; PERITONEAL-MACROPHAGES; MYCOBACTERIUM-AVIUM;
Keywords:
macrophage; host defense; nitric oxide; superoxide; peroxynitrite;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Eugster, HP Univ Zurich Hosp, Dept Internal Med, Clin Immunol Sect, Haldeliweg 4, CH-8044 Zurich, Switzerland Univ Zurich Hosp Haldeliweg 4 Zurich Switzerland CH-8044 land
Citazione:
M. Muller et al., "Reduced antilisterial activity of TNF-deficient bone marrow-derived macrophages is due to impaired superoxide production", EUR J IMMUN, 29(10), 1999, pp. 3089-3097

Abstract

Mice deficient for TNF ligand and receptor type 1 have demonstrated the importance of TNF in the host defense against Listeria monocytogenes. To investigate the particular deficiency of macrophages derived from TNF/lymphotoxin (LT)-alpha(-/-) mice in antilisterial growth control, bone marrow-derived macrophages (BMDM) were used for in vitro infection experiments. After the combined treatment with IFN-gamma and lipopolysaccharide (LPS), production of NO by wild-type (wt) and TNF/LT-alpha(-/-) BMDM was induced to comparable levels, but only wt BMDM controlled L. monocytogenes growth efficiently. Nevertheless, inhibition of NO production led to a remarkable loss of antilisterial activity. This suggests that presence of NO is necessary but notsufficient for L. monocytogenes killing and that elimination of L. monocytogenes requires additional effector molecules. The LPS-inducible superoxideproduction of TNF/LT-alpha(-/-) BMDM was impaired. Accordingly both scavenging of superoxide and peroxynitrite led to reduced L. monocytogenes killing by wt BMDM. In addition, peroxynitrite was able to kill L. monocytogenes in vitro. Together these findings suggest that the defective host defense of TNF/LT-alpha-deficient mice against L. monocytogenes partially stems fromreduced superoxide production of macrophages due to the absence of TNF andimply a function for peroxynitrite, the reaction product of NO and superoxide, in the intracellular killing of L. monocytogenes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 03:03:08