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Titolo:
Ligand-induced recruitment of a histone deacetylase in the negative-feedback regulation of the thyrotropin beta gene
Autore:
Sasaki, S; Lesoon-Wood, LA; Dey, A; Kuwata, T; Weintraub, BD; Humphrey, G; Yang, WM; Seto, E; Yen, PM; Howard, BH; Ozato, K;
Indirizzi:
NICHHD, Lab Mol Growth Regulat, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 Growth Regulat, NIH, Bethesda, MD 20892 USA NIDDKD, Clin Endocrinol Branch, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 docrinol Branch, NIH, Bethesda, MD 20892 USA Univ Hawaii, Canc Res Ctr Hawaii, Honolulu, HI 96813 USA Univ Hawaii Honolulu HI USA 96813 Res Ctr Hawaii, Honolulu, HI 96813 USA Univ Maryland, Sch Med, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 nd, Sch Med, Baltimore, MD 21201 USA Univ Maryland, Inst Human Virol, Mol Endocrinol Lab, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 ocrinol Lab, Baltimore, MD 21201 USA Univ S Florida, Dept Med Microbiol & Immunol, H Lee Moffit Canc Ctr & Res Inst, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 anc Ctr & Res Inst, Tampa, FL 33612 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 19, volume: 18, anno: 1999,
pagine: 5389 - 5398
SICI:
0261-4189(19991001)18:19<5389:LROAHD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROID-HORMONE RECEPTOR; TRANSCRIPTION FACTOR PIT-1/GHF-1; EMBRYONAL CARCINOMA-CELLS; RETINOIC ACID RECEPTOR; SUBUNIT GENE; REPRESS TRANSCRIPTION; CHROMATIN STRUCTURE; RESPONSE ELEMENT; NUCLEAR RECEPTORS; TRICHOSTATIN-A;
Keywords:
histone deacetylases; negative feedback; thyroid hormone; thyrotropin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Ozato, K NICHHD, Lab Mol Growth Regulat, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 egulat, NIH, Bethesda, MD 20892 USA
Citazione:
S. Sasaki et al., "Ligand-induced recruitment of a histone deacetylase in the negative-feedback regulation of the thyrotropin beta gene", EMBO J, 18(19), 1999, pp. 5389-5398

Abstract

We have investigated ligand-dependent negative regulation of the thyroid-stimulating hormone beta (TSH beta) gene. Thyroid hormone (T3) markedly repressed activity of the TSH beta promoter that had been stably integrated into GH(3) pituitary cells, through the conserved negative regulatory element (NRE) in the promoter. By DNA affinity binding assay, we show that the NRE constitutively binds to the histone deacetylase 1 (HDAC1) present in GH3 cells. Significantly, upon addition of T3, the NRE further recruited the thyroid hormone receptor (TR beta) and another deacetylase, HDAC2, This recruitment coincided with an alteration of in vivo chromatin structure, as revealed by changes in restriction site accessibility. Supporting the direct interaction between TR and HDAC, in vitro assays showed that TR, through its DNA binding domain, strongly bound to HDAC2, Consistent with the role for HDACs in negative regulation, an inhibitor of the enzymes, trichostatin A, attenuated T3-dependent promoter repression. We suggest that ligand-dependent histone deacetylase recruitment is a mechanism of the negative-feedback regulation, a critical function of the pituitary-thyroid axis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:17:30