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Titolo:
Electrostatic interaction between chitosan-modified latex particles and bovine serum albumin
Autore:
Chern, CS; Lee, CK; Ho, CC;
Indirizzi:
Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan Natl Taiwan Univ Sci & Technol Taipei Taiwan 106 ngn, Taipei 106, Taiwan
Titolo Testata:
COLLOID AND POLYMER SCIENCE
fascicolo: 10, volume: 277, anno: 1999,
pagine: 979 - 985
SICI:
0303-402X(199910)277:10<979:EIBCLP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADSORPTION; STABILITY; SURFACE; BSA;
Keywords:
bovine serum albumin; chitosan-modified particles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Chern, CS Natl Taiwan Univ Sci & Technol, Dept Chem Engn, 43 Keelung Rd,Sec 4, Taipei 106, Taiwan Natl Taiwan Univ Sci & Technol 43 Keelung Rd,Sec 4 Taipei Taiwan 106
Citazione:
C.S. Chern et al., "Electrostatic interaction between chitosan-modified latex particles and bovine serum albumin", COLLOID P S, 277(10), 1999, pp. 979-985

Abstract

Electrostatic interaction between poly(methyl methacrylate) latex particles with different levels of chitosan modification and bovine serum albumin (BSA) was investigated. The critical flocculation concentration is in the range 5-15 nmol dm(-3) for these latex products toward added BSA. A series ofisothermal equilibrium adsorption experiments shows that the adsorption process is divided into two distinct intervals. Adsorption of BSA on latex particles in intervals I and II is primarily controlled by charge neutralization and hydrophobic interaction, respectively. Intervals I and II can be reasonably described by an empirical parabola equation and the Langmuir isotherm model, respectively. The maximum amount of BSA adsorbed per unit weightof polymer particles was observed at pH congruent to 5. A maximum elution yield of about 80% can be achieved using NaSCN as the elution electrolyte, and NaSCN is more effective in inducing desorption of BSA from the particlesurface than NaCl. The chitosan content has very little effect on the interaction between latex particles and BSA. By contrast? the influence of the content of 2,2'-azobis(2-amidinopropane) dihydrochloride, a cationic initiator used in preparing the chitosan-modified latex products, on the BSA adsorption process is significant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:14:16