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Titolo:
Presence of donor- and recipient-derived DNA in cell-free urine samples ofrenal transplantation recipients: Urinary DNA chimerism
Autore:
Zhang, J; Tong, KL; Li, PKT; Chan, AYW; Yeung, CK; Pang, CCP; Wong, TYH; Lee, KC; Lo, YMD;
Indirizzi:
Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong Chinese Univ Hong Kong Shatin Hong Kong Chem Pathol, Shatin, Hong Kong Chinese Univ Hong Kong, Prince Wales Hosp, Dept Surg, Shatin, Hong Kong Chinese Univ Hong Kong Shatin Hong Kong p, Dept Surg, Shatin, Hong Kong Chinese Univ Hong Kong, Prince Wales Hosp, Dept Ophthalmol & Visual Sci, Shatin, Hong Kong Chinese Univ Hong Kong Shatin Hong Kong & Visual Sci, Shatin, Hong Kong Princess Margaret Hosp, Dept Med, Kowloon, Hong Kong Princess Margaret Hosp Kowloon Hong Kong , Dept Med, Kowloon, Hong Kong Princess Margaret Hosp, Dept Pathol, Kowloon, Hong Kong Princess Margaret Hosp Kowloon Hong Kong ept Pathol, Kowloon, Hong Kong Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong Prince Wales Hosp Shatin Hong Kong t Med & Therapeut, Shatin, Hong Kong
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 10, volume: 45, anno: 1999,
pagine: 1741 - 1746
SICI:
0009-9147(199910)45:10<1741:PODARD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIVER-TRANSPLANTATION; ORGAN-TRANSPLANTATION; ALLOGRAFT REJECTION; GRAFT ACCEPTANCE; MICROCHIMERISM; KIDNEY; TOLERANCE; APOPTOSIS; MIGRATION; BLADDER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Lo, YMD Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Room 38023,1-F Clin Sci Bldg,30-32 Ngan Shing St, Shatin, Hong Kong Chinese UnivHong Kong Room 38023,1-F Clin Sci Bldg,30-32 Ngan Shing St Shatin Hong Kong
Citazione:
J. Zhang et al., "Presence of donor- and recipient-derived DNA in cell-free urine samples ofrenal transplantation recipients: Urinary DNA chimerism", CLIN CHEM, 45(10), 1999, pp. 1741-1746

Abstract

Background: Previous studies have indicated that microchimerism is presentin body tissues, peripheral blood, and plasma of recipients after organ transplantation. We hypothesize that donor-derived DNA may also be present incell-free urine of renal transplant recipients and that the concentrationsof urine DNA may be correlated with graft rejection. Methods: Thirty-one female patients who had renal transplantation were enrolled in the study. In women with male organ donors, the SRY gene on the Y chromosome was used as a marker for donor-derived DNA. Real-time quantitative PCR for the SRY and beta-globin genes was carried out on cell-free urinary DNA from these patients. Serial urine samples from a female renal transplant recipient undergoing an acute rejection episode were also collected and analyzed with the beta-globin quantitative PCR system. Results: SRY sequences were detected in the urine of 14 of 17 female patients with male organ donors. None of the 14 patients with female organ donors had detectable SRY sequences in urinary DNA. The median fractional concentration of donor-derived DNA was 8.7% (interquartile range, 1.9-26.4%). During the acute rejection episode, urinary concentrations of the beta-globin gene were markedly increased, with the concentrations re-turning rapidly tonormal following antirejection treatment. Conclusions: Our results demonstrate that urinary DNA chimerism is presentfollowing renal transplantation. The measurement of urinary DNA using quantitative PCR may be useful for the diagnosis and monitoring of graft rejection. (C) 1999 American Association for Clinical Chemistry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:39:23