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Titolo:
Pituitary adenylate cyclase activating polypeptide (PACAP) in the rat mammary gland
Autore:
Skakkebaek, M; Hannibal, J; Fahrenkrug, J;
Indirizzi:
Univ Copenhagen, Bispebjerg Hosp, Dept Clin Biochem, DK-2400 Copenhagen NV, Denmark Univ Copenhagen Copenhagen Denmark NV em, DK-2400 Copenhagen NV, Denmark
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 298, anno: 1999,
pagine: 153 - 159
SICI:
0302-766X(199910)298:1<153:PACAP(>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; MESSENGER-RNA; NERVE-FIBERS; EXPRESSION; CAPSAICIN; CELLS; IMMUNOREACTIVITY; LOCALIZATION; TRACT; RECEPTORS;
Keywords:
co-existence neuropeptides (PACAP, VIP, CGRP); radioimmunoassay; immunohistochemistry; sensory nerves; rat (Wistar);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Fahrenkrug, J Univ Copenhagen, Bispebjerg Hosp, Dept Clin Biochem, DK-2400Copenhagen NV, Denmark Univ Copenhagen Copenhagen Denmark NV penhagen NV, Denmark
Citazione:
M. Skakkebaek et al., "Pituitary adenylate cyclase activating polypeptide (PACAP) in the rat mammary gland", CELL TIS RE, 298(1), 1999, pp. 153-159

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP), a member of the vasoactive intestinal polypeptide (VIP) family of peptides, is present inthe brain and in neuronal elements of a number of peripheral organs. Sinceno information on PACAP in the mammary gland exists, we have investigated,by radioimmunoassay and immunohistochemistry, the occurrence and distribution of PACAP immunoreactivity in the mammary gland of lactating and non-lactating rats. A specific monoclonal mouse anti-PACAP antibody has been used to show that the peptide is located in nerve fibres associated with bundlesof circular and longitudinal smooth muscle surrounding the lactiferous duct of the nipple. PACAP-immunoreactive nerve fibres and nerve bundles are present in the subepidermal connective tissue of the nipple and in the mammary parenchyma, some of the fibres being in close contact with blood vessels. Occasionally, a few delicate varicose fibres are associated with secretoryalveoli and lactiferous ducts. The majority of PACAP-positive nerve fibresare, however, located in the glabrous skin of the nipple and the hairy skin adjacent to the nipple forming a subepithelial plexus from which delicatevaricose nerve fibres enter the overlying epithelium. Double immunostaining for PACAP and a marker for sensory neurons, calcitonin gene-related peptide, has disclosed that the two peptides are almost completely colocalized. A minor population of the PACAP-immunoreactive nerve fibres shows co-existence with VIP. Although no obvious changes at the immunohistochemical level could be observed during pregnancy or lactation, elevated concentrations ofimmunoreactive PACAP-38 in mammary extracts have been found during lactation. Our data suggest that PACAP is involved in the nervous control of mammary gland function, probably in the transmission of suckling stimuli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:33:28