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Titolo:
Unique differentiation programs of human fetal liver stem cells shown bothin vitro and in vivo in NOD/SCID mice
Autore:
Nicolini, FE; Holyoake, TL; Cashman, JD; Chu, PPY; Lambie, K; Eaves, CJ;
Indirizzi:
British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy Vancouver BC Canada V5Z 1L3 BC V5Z 1L3, Canada Univ British Columbia, Dept Med Genet, Vancouver, BC, Canada Univ British Columbia Vancouver BC Canada d Genet, Vancouver, BC, Canada
Titolo Testata:
BLOOD
fascicolo: 8, volume: 94, anno: 1999,
pagine: 2686 - 2695
SICI:
0006-4971(19991015)94:8<2686:UDPOHF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT BONE-MARROW; HUMAN HEMATOPOIETIC-CELLS; ERYTHROID COLONY FORMATION; UMBILICAL-CORD BLOOD; EXPANSION IN-VITRO; LONG-TERM; PROGENITOR CELLS; FUNCTIONAL-CHARACTERIZATION; MULTILINEAGE HEMATOPOIESIS; REPOPULATING ABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Eaves, CJ British Columbia Canc Agcy, Terry Fox Lab, 601 W 10th Ave, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy 601 W 10th Ave Vancouver BC Canada V5Z 1L3
Citazione:
F.E. Nicolini et al., "Unique differentiation programs of human fetal liver stem cells shown bothin vitro and in vivo in NOD/SCID mice", BLOOD, 94(8), 1999, pp. 2686-2695

Abstract

Comparative measurements of different types of hematopoietic progenitors present in human fetal liver, cord blood, and adult marrow showed a large (up to 250-fold), stage-specific, but lineage-unrestricted, amplification of the colony-forming cell (CFC) compartment in the fetal liver, with a higherratio of all types of CFC to long-term culture-initiating cells (LTC-IC) and a lower ratio of total (mature) cells to CFC. Human fetal liver LTC-IC were also found to produce more CFC in LTC than cord blood or adult marrow LTC-IC, and more of the fetal liver LTC-IC-derived CFC were erythroid. Humanfetal liver cells regenerated human multilineage hematopoiesis in NOD/SCIDmice with the same kinetics as human cord blood and adult marrow cells, but sustained a high level of terminal erythropoiesis not seen in adult marrow-engrafted mice unless exogenous human erythropoietin (Epo) was injected. This may be due to a demonstrated 10-fold lower activity of murine versus human Epo on human cells, sufficient to distinguish between a differential Epo sensitivity of fetal and adult erythroid precursors. Examination of human LTC-IC, CFC, and erythroblasts generated either in NOD/SCID mice and/or in LTC showed the types of cells and hemoglobins produced also to reflect their ontological origin, regardless of the environment in which the erythroid precursors were generated. We suggest that ontogeny may affect the behavior of cells at many stages of hematopoietic cell differentiation through key changes in shared signaling pathways. (C) 1999 by The American Society ofHematology.

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Documento generato il 21/10/20 alle ore 16:26:40