Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The N-terminal half of NPM dissociates from nucleoli of HeLa cells after anticancer drug treatments
Autore:
Chan, PK; Bloom, DA; Hoang, TT;
Indirizzi:
Baylor Coll Med, Dept Pharmacol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Pharmacol, Houston, TX 77030 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 264, anno: 1999,
pagine: 305 - 309
SICI:
0006-291X(19991014)264:1<305:TNHOND>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE TUMOR-CELLS; PROTEIN-B23 TRANSLOCATION; NUCLEOPHOSMIN/B23; LOCALIZATION; LYMPHOMA; FUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Chan, PK Baylor Coll Med, Dept Pharmacol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 acol, Houston, TX 77030 USA
Citazione:
P.K. Chan et al., "The N-terminal half of NPM dissociates from nucleoli of HeLa cells after anticancer drug treatments", BIOC BIOP R, 264(1), 1999, pp. 305-309

Abstract

NPM (nucleophosmin/B23) is a nucleolar phosphoprotein abundant in tumor cells. It dissociates from nucleoli of cells after treatments with various anticancer drugs, To determine the domain of NPM responsible for nucleolar binding, the N- and C-terminal halves of NPM were fused to GFP (green fluorescent protein) and introduced into HeLa cells. The N-terminal half (aa 1-150) of NPM (GFP-NPMN) was found localized in the nucleoli, A stable transformant of GFP-NPMN in HeLa cells was prepared and tested for association to nucleoli after anticancer drug treatments. GFP-NPMN dissociates from nucleoliafter treatments with daunomycin, actinomycin D, camptothecin, and toyocamycin, The dissociation is time- and dose-dependent, and correlates with thecytotoxicity induced by the drugs. These results indicate that a stable transformant of GFP-NPMN in HeLa cells may be useful for the screening of anticancer drugs. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:51:21