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Titolo:
Flow-cytometric analysis of leukocyte alkaline phosphatase in myelodysplastic syndromes
Autore:
Okamoto, T; Okada, M; Yamada, S; Fujimori, Y; Takemoto, Y; Masuhara, K; Kakishita, E;
Indirizzi:
Hyogo Coll Med, Dept Internal Med 2, Nishinomiya, Hyogo 6638501, Japan Hyogo Coll Med Nishinomiya Hyogo Japan 6638501 miya, Hyogo 6638501, Japan Osaka Kosei Nenkin Hosp, Dept Orthoped Surg, Osaka, Japan Osaka Kosei Nenkin Hosp Osaka Japan p, Dept Orthoped Surg, Osaka, Japan
Titolo Testata:
ACTA HAEMATOLOGICA
fascicolo: 2, volume: 102, anno: 1999,
pagine: 89 - 93
SICI:
0001-5792(1999)102:2<89:FAOLAP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; HEALTHY-VOLUNTEERS; CLONAL ANALYSIS; NEUTROPHILS; CELLS; EXPRESSION; CHROMOSOME;
Keywords:
flow cytometry; granulocyte colony-stimulating factor; leukocyte alkaline phosphatase; myelodysplastic syndromes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Okamoto, T Hyogo Coll Med, Dept Internal Med 2, 1-1 Mukogawa Cho, Nishinomiya, Hyogo 6638501, Japan Hyogo Coll Med 1-1 Mukogawa Cho Nishinomiya HyogoJapan 6638501
Citazione:
T. Okamoto et al., "Flow-cytometric analysis of leukocyte alkaline phosphatase in myelodysplastic syndromes", ACT HAEMAT, 102(2), 1999, pp. 89-93

Abstract

The expression of leukocyte alkaline phosphatase (LAP) in neutrophils is reduced in some patients with myelodysplastic syndrome (MDS), We quantitatively assayed for LAP in MDS leukocytes by a flow cytometry based method using a monoclonal antibody raised against human bone alkaline phosphatase. TheLAP expression was assayed in blood samples from a group of 46 MRS patients, consisting of 39 patients with refractory anemia (RA), 3 with RA with excess blasts (RAEB), and 4 patients with RAEB in transformation. The percentage of LAP-positive cells was significantly higher in the MDS patients thanin the normal subjects and also higher in IRA than in RAEB and RAEB in transformation. To investigate the cause of the elevated LAP expression, we measured the serum concentrations of several cytokines. The granulocyte colony-stimulating factor (G-CSF) level was significantly elevated in MDS patients, and the serum G-CSF concentration clearly correlated with the percentage of LAP-positive cells. Thus, the LAP activity in RA is higher than in normal subjects, and G-CSF is thought to be one of the causes stimulating LAP expression in MDS neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 12:52:13