Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Repair of radiation-induced DNA strand breaks does not occur preferentially in transcriptionally active DNA
Autore:
Ljungman, M;
Indirizzi:
Univ Michigan, Ctr Comprehens Canc, Dept Radiat Oncol, Div Canc Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 v Canc Biol, Ann Arbor, MI 48109 USA
Titolo Testata:
RADIATION RESEARCH
fascicolo: 4, volume: 152, anno: 1999,
pagine: 444 - 449
SICI:
0033-7587(199910)152:4<444:RORDSB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; IONIZING-RADIATION; DHFR GENE; CHROMATIN STRUCTURE; COUPLED REPAIR; IN-VIVO; TRANSCRIBED STRAND; TORSIONAL TENSION; COCKAYNE-SYNDROME; INDUCED DAMAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ljungman, M Univ Michigan, Ctr Comprehens Canc, Dept Radiat Oncol, Div Canc Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Ann Arbor, MI 48109 USA
Citazione:
M. Ljungman, "Repair of radiation-induced DNA strand breaks does not occur preferentially in transcriptionally active DNA", RADIAT RES, 152(4), 1999, pp. 444-449

Abstract

Certain DNA base lesions induced by ionizing radiation or oxidative stressare repaired faster from the transcribed strand of active genes compared to the genome overall, In this study, it was investigated whether radiation-induced DNA strand breaks are preferentially repaired in active genes compared to the genome as a whole in CHO cells. The alkaline unwinding techniquecoupled to slot-blot hybridization with specific DNA probes was used to study the induction and repair of DNA strand breaks in defined DNA sequences. Results using this technique showed a linear dose response for the formation of radiation-induced DNA strand breaks in the dihydrofolate reductase (DHPR) gene. Furthermore, the half-life of radiation-induced strand breaks was less than 5 min in the DHFR gene, in the ribosomal genes, and in the genome as a whole. These results suggest that the repair of DNA strand breaks is fast and uniform in the genome of mammalian cells, (C) 1999 by Radiation Research Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:03:32