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Titolo:
Affinity purified human antiphospholipid antibodies bind normal term placenta
Autore:
Donohoe, S; Kingdom, JCP; Mackie, IJ;
Indirizzi:
Univ Coll London, Dept Haematol, London WC1E 6HX, England Univ Coll London London England WC1E 6HX matol, London WC1E 6HX, England Univ Coll London, Dept Obstet & Gynaecol, London, England Univ Coll London London England Dept Obstet & Gynaecol, London, England
Titolo Testata:
LUPUS
fascicolo: 7, volume: 8, anno: 1999,
pagine: 525 - 531
SICI:
0961-2033(1999)8:7<525:APHAAB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; ANTICARDIOLIPIN ANTIBODIES; DECIDUAL VASCULOPATHY; FETAL LOSS; PREGNANCY; CELLS; WOMEN; ANTICOAGULANT; THROMBOSIS; EXPRESSION;
Keywords:
antiphospholipid antibodies; placental proteins; immunofluorescence; beta(2)-glycoprotein-I;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Donohoe, S Univ Coll London, Dept Haematol, London WC1E 6HX, England Univ Coll London London England WC1E 6HX on WC1E 6HX, England
Citazione:
S. Donohoe et al., "Affinity purified human antiphospholipid antibodies bind normal term placenta", LUPUS, 8(7), 1999, pp. 525-531

Abstract

Antiphospholipid antibodies (aPL) are associated with an increased incidence of fetal loss, but the pathophysiology remains unclear. One mechanism may involve the binding of aPL directly to the placenta where they may initiate placental thrombosis and infarction. We have developed an immunofluorescent technique to detect human aPL binding to human placenta. Endogenous immunoglobulins were eluted by extensive washing and residual staining was prevented by incorporating multiple blocking steps. APL were affinity purifiedon both cardiolipin and phosphatidylserine liposomes from the sera of six patients with aPL (five antiphospholipid syndrome (,APS) patients and one post bone marrow transplant patient). Heterogeneous binding to normal term placenta, involving either the trophoblast microvillous surface, stromal andperivascular regions was demonstrated by affinity purified aPL from five of six patients. Preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting studies have demonstrated that aPL bind a number of placental proteins. beta(2)GPI was not the predominantprotein bound by aPL using this technique. This study provides further evidence for the involvement of aPL in mediating placental damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:56:36