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Titolo:
Monitoring of minimal residual leukemia in patients with MLL-AF9 positive acute myeloid leukemia by RT-PCR
Autore:
Mitterbauer, G; Zimmer, C; Fonatsch, C; Haas, OA; Thalhammer-Scherrer, R; Schwarzinger, I; Kahls, P; Jaeger, U; Lechner, K; Mannhalter, C;
Indirizzi:
Univ Vienna, Sch Med, Dept Lab Med, Div Mol Biol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 , Div Mol Biol, A-1090 Vienna, Austria Univ Vienna, Dept Lab Med, Div Hematol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 d, Div Hematol, A-1090 Vienna, Austria Univ Vienna, Dept Med 1, Div Hematol & Hemostaseol, A-1090 Vienna, AustriaUniv Vienna Vienna Austria A-1090 & Hemostaseol, A-1090 Vienna, Austria Univ Vienna, Dept Med 1, Bone Marrow Transplantat Unit, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 nsplantat Unit, A-1090 Vienna, Austria Univ Vienna, Inst Med Biol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Inst Med Biol, A-1090 Vienna, Austria St Anna Childrens Hosp, Childrens Canc Res Inst, A-1090 Vienna, Austria StAnna Childrens Hosp Vienna Austria A-1090 nst, A-1090 Vienna, Austria
Titolo Testata:
LEUKEMIA
fascicolo: 10, volume: 13, anno: 1999,
pagine: 1519 - 1524
SICI:
0887-6924(199910)13:10<1519:MOMRLI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; FUSION MESSENGER-RNA; MLL GENE; CHROMOSOME-TRANSLOCATION; 11Q23 TRANSLOCATIONS; DROSOPHILA-TRITHORAX; ALL-1 GENE; FUSES; REARRANGEMENTS; IDENTIFICATION;
Keywords:
acute myeloid leukemia; t(9;11)(p22;q23); MLL-AF9; RT-PCR; minimal residual disease (MRD);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Mannhalter, C Univ Vienna, Sch Med, Dept Lab Med, Div Mol Biol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 ia
Citazione:
G. Mitterbauer et al., "Monitoring of minimal residual leukemia in patients with MLL-AF9 positive acute myeloid leukemia by RT-PCR", LEUKEMIA, 13(10), 1999, pp. 1519-1524

Abstract

Twenty-seven patients with AML and MLL gene rearrangement were analyzed bya reverse transcriptase polymerase chain reaction (RT-PCR) for the MLL-AF9translocation. The MLL-AF9 fusion transcript was detected in six patients. In five patients, the breakpoint of the AF9 gene was located within the recently described site A; in one patient, a novel breakpoint (AF9 site D) mapped to a position 377 bp 3' of site A. Five patients could be serially monitored for a period of 4-23 months. Two patients became two-step PCR negative in bone marrow and peripheral blood. Molecular remission was achieved rapidly after one cycle of induction chemotherapy. Both patients are in continuous complete remission (CR) at 22 and 15 months, respectively. Two patients who had achieved hematological CR did not become PCR negative and MLL-AF9 fusion transcripts were detectable in all samples after induction and consolidation chemotherapy. One patient relapsed 5 months after achieving CR. The other patient received allogeneic bone marrow transplantation from an HLA-identical sibling 2 months after achieving hematological CR and became PCR negative 4 weeks after transplantation. In the fifth patient, hematological CR could not be achieved with two cycles of intensive induction chemotherapy, and MLL-AF9 transcripts were present in all samples tested. Our dataindicate that MLL-AF9 RT-PCR is specific for the t(9;11) translocation. PCR negativity can be achieved in responding patients already 1 month after induction chemotherapy. The fast reduction of MLL-AF9 positive blast cells below the detection limit of RT-PCR seems to be a prerequisite for long-termCR. The results of RT-PCR may be useful for treatment decisions (eg BMT).

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Documento generato il 05/12/20 alle ore 01:11:37