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Titolo:
Modulation of sulfate renal transport by alterations in cell membrane fluidity
Autore:
Lee, HJ; Balasubramanian, SV; Murer, H; Biber, J; Morris, ME;
Indirizzi:
SUNY Buffalo, Sch Pharm, Dept Pharmaceut, Amherst, NY 14260 USA SUNY Buffalo Amherst NY USA 14260 Dept Pharmaceut, Amherst, NY 14260 USA Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 siol, CH-8057 Zurich, Switzerland
Titolo Testata:
JOURNAL OF PHARMACEUTICAL SCIENCES
fascicolo: 10, volume: 88, anno: 1999,
pagine: 976 - 980
SICI:
0022-3549(199910)88:10<976:MOSRTB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-GLUCOSE TRANSPORT; LIPID-COMPOSITION; EPITHELIAL-CELLS; INORGANIC SULFATE; BENZYL ALCOHOL; RAT; CHOLESTEROL; VESICLES; COTRANSPORT; QUANTITATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, ME SUNY Buffalo, Sch Pharm, Dept Pharmaceut, Amherst, NY 14260 USASUNY Buffalo Amherst NY USA 14260 aceut, Amherst, NY 14260 USA
Citazione:
H.J. Lee et al., "Modulation of sulfate renal transport by alterations in cell membrane fluidity", J PHARM SCI, 88(10), 1999, pp. 976-980

Abstract

Changes in membrane fluidity have been shown to alter the sodium-dependentrenal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-SI). Preincubation of cells with 0.2 mM cholesterol significantly decreased the V-max for sodium/sulfate cotransport (13.69 +/- 1.11 vs 10.15 +/- 1.17 nmol/mg protein/5 min, mean +/- SD, n = 4, p <0.01) with no significant alteration in K-m. The addition of benzyl alcohol (20 mM) to cells increased the V-max of sulfate uptake by 20% (11.97 +/- 0.91 vs 14.35 +/- 0.56 nmol/mg protein/5 min, mean +/- SD, n = 3, p < 0.05)with no significant change in Km. Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.

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Documento generato il 11/07/20 alle ore 20:24:22