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Titolo:
High-speed CCD imaging system for monitoring neural activity in vivo and in vitro, using a voltage-sensitive dye
Autore:
Takashima, I; Ichikawa, M; Iijima, T;
Indirizzi:
Electrotech Lab, Supermol Sci Div, Tsukuba, Ibaraki 3058568, Japan Electrotech Lab Tsukuba Ibaraki Japan 3058568 uba, Ibaraki 3058568, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1-2, volume: 91, anno: 1999,
pagine: 147 - 159
SICI:
0165-0270(19990915)91:1-2<147:HCISFM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALAMANDER OLFACTORY-BULB; OPTICAL-RECORDING SYSTEM; FUNCTIONAL-ORGANIZATION; ELECTRICAL-ACTIVITY; VISUAL-CORTEX; NEURONS; POTENTIALS; CAMERA; ARRAY;
Keywords:
optical imaging; CCD; voltage-sensitive dye; epileptiform discharge; somatosensory cortex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Iijima, T Electrotech Lab, Supermol Sci Div, 1-1-4 Umezono, Tsukuba, Ibaraki 3058568, Japan Electrotech Lab 1-1-4 Umezono Tsukuba Ibaraki Japan 3058568 apan
Citazione:
I. Takashima et al., "High-speed CCD imaging system for monitoring neural activity in vivo and in vitro, using a voltage-sensitive dye", J NEUROSC M, 91(1-2), 1999, pp. 147-159

Abstract

We have designed and constructed a high-speed CCD imaging system for optically detecting neural activity from preparations stained externally with a voltage-sensitive dye, and have used this system to image evoked and epileptiform neural activity in the rat somatosensory cortex. The imaging system uses a commercially available 1/3-in. CCD chip, and it can continuously capture images for more than 8 s, at 1000 frames/s, with a spatial resolution of 128 x 62 pixels. The spatial/temporal resolution of the CCD sensor is variable by changing the geometry of on-chip binning pixels, which can be controlled by a PC/AT computer. Dye bleaching correction was not necessary forlong-term imaging of epileptiform neural events, since the sensitivity of the CCD sensor was increased by combining the signal from adjacent pixels. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:52:02