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Titolo:
Ion-pair reversed-phase HPLC determination of creatinine in urine
Autore:
Resines, JA; Arin, MJ; Diez, MT; del Moral, PG;
Indirizzi:
Univ Leon, Fac Biol, Dept Bioquim & Biol Mol, E-24071 Leon, Spain Univ Leon Leon Spain E-24071 ept Bioquim & Biol Mol, E-24071 Leon, Spain Univ Leon, Fac Biol, Dept Fis Quim & Expres Graf, E-24071 Leon, Spain UnivLeon Leon Spain E-24071 Fis Quim & Expres Graf, E-24071 Leon, Spain
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 16, volume: 22, anno: 1999,
pagine: 2503 - 2510
SICI:
1082-6076(1999)22:16<2503:IRHDOC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE-LIQUID-CHROMATOGRAPHY; ULTRAVIOLET DETECTION; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Resines, JA Univ Leon, Fac Biol, Dept Bioquim & Biol Mol, Campus Vegazana,E-24071 Leon, Spain Univ Leon Campus Vegazana Leon Spain E-24071 4071 Leon, Spain
Citazione:
J.A. Resines et al., "Ion-pair reversed-phase HPLC determination of creatinine in urine", J LIQ CHR R, 22(16), 1999, pp. 2503-2510

Abstract

A high performance liquid chromatography (HPLC) with isocratic ion-pair-reversed-phase separation and UV detection at 230 nm is proposed for the determination of creatinine in urine. The analysis is achieved on a C-18-Symmetry column (4 mu m, 150mm x 3.9mm ID) using a mixture of 10 mM phosphate buffer pH=4, containing 3mM 1-octane-sulfonic acid, sodium salt and methanol (97:3). A flow-rate of 0.5mL/min and a column temperature of 30 degrees C were employed. We validated the method for linearity, precision, and accuracyand the results were found to be excellent. The procedure proposed here isan alternative to less specific colorimetric methods.

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Documento generato il 19/01/20 alle ore 09:51:47