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Titolo:
A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis
Autore:
Chung, JY; Bahk, YY; Huh, S; Kang, SY; Kong, Y; Cho, SY;
Indirizzi:
Sung Kyun Kwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 , Suwon 440746, South Korea Hallym Univ, Coll Med, Dept Parasitol, Chuncheon, South Korea Hallym UnivChuncheon South Korea ept Parasitol, Chuncheon, South Korea Chung Ang Univ, Coll Med, Dept Parasitol, Seoul 156756, South Korea Chung Ang Univ Seoul South Korea 156756 sitol, Seoul 156756, South Korea
Titolo Testata:
JOURNAL OF INFECTIOUS DISEASES
fascicolo: 4, volume: 180, anno: 1999,
pagine: 1307 - 1315
SICI:
0022-1899(199910)180:4<1307:AR1POT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOELECTROTRANSFER BLOT; BOVINE CYSTICERCOSIS; IMMUNE-RESPONSE; MAJOR CAUSE; CYST FLUID; ANTIGEN; CRASSICEPS; COMMUNITY; MEXICO; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Kong, Y Sung Kyun Kwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 440746, South Korea
Citazione:
J.Y. Chung et al., "A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis", J INFEC DIS, 180(4), 1999, pp. 1307-1315

Abstract

Neurocysticercosis (NCC) is an important cause of neurological disease worldwide. A 10-kDa antigen of Taenia solium metacestodes (TsMs) has been shown to be specific for immunodiagnosis of NCC, Screening of a TsM complementary DNA (cDNA) library isolated a cDNA encoding this protein. The cloned cDNA contained a 258-bp complete open-reading frame that encodes an 86-amino acid polypeptide with a calculated molecular weight of 9582 Da. It showed 73% homology with a 10-kDa antigen of T. crassiceps. The recombinant protein was expressed bacterially as a fusion protein at a high level. In immunoblot with recombinant protein, 97% (184/190) of sera from patients with activeNCC showed strong reactivity, whereas 14% (4/29) of those from patients with chronic calcified NCC reacted weakly. In 180 sera from other patients with parasitic infections and from normal controls, it showed 98% specificityA single recombinant TsM antigen has a high potential for serological differentiation of active NCC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:31:09