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Titolo:
Specificity of CTL interactions with peptide-MHC class I tetrameric complexes is temperature dependent
Autore:
Whelan, JA; Dunbar, PR; Price, DA; Purbhoo, MA; Lechner, F; Ogg, GS; Griffiths, G; Phillips, RE; Cerundolo, V; Sewell, AK;
Indirizzi:
Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX3 9DU, England Univ Oxford Oxford England OX3 9DU ept Clin Med, Oxford OX3 9DU, England John Radcliffe Hosp, Inst Mol Med, Mol Immunol Grp, Oxford OX3 9DU, England John Radcliffe Hosp Oxford England OX3 9DU Grp, Oxford OX3 9DU, England Sir William Dunn Sch, Dept Pathol, Oxford, England Sir William Dunn Sch Oxford England n Sch, Dept Pathol, Oxford, England
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 8, volume: 163, anno: 1999,
pagine: 4342 - 4348
SICI:
0022-1767(19991015)163:8<4342:SOCIWP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTES; DIRECT VISUALIZATION; VIRAL-INFECTION; CELL RECEPTORS; RHESUS-MONKEYS; VACCINIA VIRUS; RESPONSES; RECOGNITION; FREQUENCY; REQUIRES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sewell, AK Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Room 7508, Oxford OX3 9DU, England Univ Oxford Room 7508 Oxford England OX3 9DU OX3 9DU, England
Citazione:
J.A. Whelan et al., "Specificity of CTL interactions with peptide-MHC class I tetrameric complexes is temperature dependent", J IMMUNOL, 163(8), 1999, pp. 4342-4348

Abstract

Tetrameric peptide-MHC class I complexes ("tetramers") are proving invaluable as reagents for characterizing immune responses involving CTLs, However, because the TCR can exhibit a degree of promiscuity for binding peptide-MHC class I ligands, there is potential for cross-reactivity, Recent reportsshowing that the TCR/peptide-MHC interaction is dramatically dependent upon temperature led us to investigate the effects of incubation temperature on tetramer staining, We find that tetramers rapidly stain CTLs with high intensity at 37 degrees C, We examine the fine specificity of tetramer staining using a well-characterized set of natural epitope variants. Peptide variants that elicit little or no functional cellular response from CTLs can stain these cells at 4 degrees C but not at 37 degrees C when incorporated into tetramers, These results suggest that some studies reporting tetramer incubations at 4 degrees C could detect cross-reactive populations of CTLs with minimal avidity for the tetramer peptide, especially in the tetramer-lowpopulation. For identifying specific CTLs among polyclonal cell populations such as PBLs, incubation with tetramers at 37 degrees C improves the staining intensity of specific CTLs, resulting in improved separation of tetramer-high CD8(+) cells, Confocal microscopy reveals that tetramers incubated at 37 degrees C can be rapidly internalized by specific CTLs into vesicles that overlap with the early endocytic compartment, This TCR-specific internalization suggests that coupling of tetramers or analogues with toxins, which are activated only after receptor internalization, may create immunotoxins capable of killing CTLs of single specificities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:31:20