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Titolo:
Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization
Autore:
Chan, YW; Tung, WL; Griffith, M; Chow, KC;
Indirizzi:
Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong Hong Kong Univ Sci & Technol Kowloon Hong Kong chem, Kowloon, Hong Kong Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ol, Waterloo, ON N2L 3G1, Canada
Titolo Testata:
JOURNAL OF EXPERIMENTAL BOTANY
fascicolo: 339, volume: 50, anno: 1999,
pagine: 1627 - 1628
SICI:
0022-0957(199910)50:339<1627:COACET>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; EXPRESSION;
Keywords:
low molecular weight thaumatin-like protein; antifungal activity; pathogenesis-related protein; winter rye; Secale cereale;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Chow, KC Hong Kong Univ Sci & Technol, Dept Biochem, Clear Water Bay, Kowloon, HongKong Hong Kong Univ Sci & Technol Clear Water Bay Kowloon Hong Kong
Citazione:
Y.W. Chan et al., "Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization", J EXP BOT, 50(339), 1999, pp. 1627-1628

Abstract

A cDNA clone, WRTLP2, encoding an open reading frame of 173 amino acids, was recovered from a cDNA library of winter rye (Secale cereale L. Musketeer). The amino acid sequence deduced from the cloned cDNA exhibits very high sequence similarity (70-95%) with those of extracellular and low molecular weight thaumatin-like proteins of other cereals. It was possible to overexpress this isolated cDNA in Escherichia coli and it was found that the encoded protein of this clone exhibited antifungal activities against several fungal strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 08:24:42