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Titolo:
Construct-ion of a normalized cDNA library for the Trypanosoma cruzi genome project
Autore:
Urmenyi, TP; Bonaldo, MF; Soares, MB; Rondinelli, E;
Indirizzi:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21949900 BCeiro, Brazil Univ Iowa, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 wa, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa, Dept Physiol & Biophys, Iowa City, IA 52242 USA Univ Iowa IowaCity IA USA 52242 ysiol & Biophys, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
fascicolo: 5, volume: 46, anno: 1999,
pagine: 542 - 544
SICI:
1066-5234(199909/10)46:5<542:COANCL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSED SEQUENCE TAGS; GENE DISCOVERY; IDENTIFICATION;
Keywords:
expressed sequence tag; normalization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Rondinelli, E Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Cidade Univ, BR-21949900 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Cidade Univ Rio De Janeiro Brazil BR-21949900 BC
Citazione:
T.P. Urmenyi et al., "Construct-ion of a normalized cDNA library for the Trypanosoma cruzi genome project", J EUKAR MIC, 46(5), 1999, pp. 542-544

Abstract

Sequencing of the Trypanosoma cruzi genome is underway. Expressed sequencetags, obtained from cDNA libraries, facilitate mapping and gene discovery. The efficiency of large-scale generation of such tags is increased when using normalized cDNA libraries, where the frequency of individual clones is brought within a narrow range. Repetitive sequencing of abundant clones is therefore minimized. We constructed a normalized cDNA library from epimastigotes of clone CL Brener, and the efficiency of normalization of representative clones was assessed and shown to be adequate. The normalized cDNA library has been distributed to several groups and large-scale sequencing is currently in progress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:44:08