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Titolo:
Localization and changes in NADPH-diaphorase reactivity and nitric oxide synthase immunoreactivity in rat pulp following tooth preparation
Autore:
Law, AS; Baumgardner, KR; Meller, ST; Gebhart, GF;
Indirizzi:
Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Endodont, Ann Arbor, MI 48109 USA Univ Iowa, Dept Endodont, Iowa City, IA USA Univ Iowa Iowa City IA USAUniv Iowa, Dept Endodont, Iowa City, IA USA Univ Iowa, Dept Pharmacol, Iowa City, IA USA Univ Iowa Iowa City IA USAUniv Iowa, Dept Pharmacol, Iowa City, IA USA
Titolo Testata:
JOURNAL OF DENTAL RESEARCH
fascicolo: 10, volume: 78, anno: 1999,
pagine: 1585 - 1595
SICI:
0022-0345(199910)78:10<1585:LACINR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; P-LIKE IMMUNOREACTIVITY; SMOOTH-MUSCLE CELLS; DENTAL-PULP; SUBSTANCE-P; ENDOTHELIAL-CELLS; MOLECULAR-CLONING; MESSENGER-RNA; NERVE-FIBERS; GUINEA-PIG;
Keywords:
pulpal inflammation; NADPH-diaphorase; nitric oxide synthase; nitric oxide; blood vessel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Baumgardner, KR Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, 1011 N Univ St, Ann Arbor, MI 48109 USA Univ Michigan 1011 N Univ St Ann Arbor MI USA 48109 09 USA
Citazione:
A.S. Law et al., "Localization and changes in NADPH-diaphorase reactivity and nitric oxide synthase immunoreactivity in rat pulp following tooth preparation", J DENT RES, 78(10), 1999, pp. 1585-1595

Abstract

Inflammatory changes in the dental pulp are accompanied by release of a wide variety of chemical mediators. Nitric oxide, an oxidative free radical produced by the enzyme nitric oxide synthase (NOS), has been implicated in multiple inflammatory processes, which makes it a suitable marker for changes which likely occur following tooth pulp insult. Since limited informationon nitric oxide in the pulp is available, it is necessary first to examinerelative distributions of NOS in uninflamed and inflamed rat pulp. We accomplished this by characterizing regions of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity and the distribution of both macrophage NOS (macNOS) and neuronal NOS (nNOS) immunoreactivity in normal and inflamed rat molar pulp at multiple time points. The results showed that: (1) deep cavity preparation on the mesial surface of the molar produced a time-dependent inflammation, with acute inflammation early progressing to chronic, granulomatous inflammation with necrosis later that spread preferentially down the mesial root; (2) control (non-prepared) teeth showed a relatively faint and homogeneous distribution of NADPH-d and macNOS reactivity but no discernible nNOS reactivity; (3) inflamed teeth displayed localized increased intensity of NADPH-d and macNOS reactivity surrounding the inflamedarea of pulp, but no increased nNOS activity; (4) pulp vessels supplying the inflamed area showed increased NADPH-d reactivity, but no increased macNOS or nNOS reactivity; and (5) neither NADPH-d, macNOS, nor nNOS reactivitywas observed in pulpal nerves. Therefore, nitric oxide may mediate the pulpal inflammatory response through its effects on the paralesional pulp tissue and surrounding endothelial/vascular structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:28:35