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Titolo:
Basonuclin is associated with the ribosomal RNA genes on human keratinocyte mitotic chromosomes
Autore:
Tseng, H; Biegel, JA; Brown, RS;
Indirizzi:
Childrens Hosp Philadelphia, Dept Dermatol, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Childrens Hosp Philadelphia, Ctr Canc, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Childrens Hosp Philadelphia, Div Human Genet, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Univ Penn, Sch Med, Dept Pediat, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 pt Pediat, Philadelphia, PA 19104 USA Childrens Hosp, Howard Hughes Med Inst, Mol Med Lab, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 nst, Mol Med Lab, Boston, MA 02115 USA Harvard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 vard Univ, Sch Med, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 18, volume: 112, anno: 1999,
pagine: 3039 - 3047
SICI:
0021-9533(199909)112:18<3039:BIAWTR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL-KERATINOCYTES; BETA-SATELLITE DNA; RDNA TRANSCRIPTION; TOPOISOMERASE-II; POLYMERASE-I; PROTEIN; LOCALIZATION; IDENTIFICATION; ORGANIZATION; MUTAGENESIS;
Keywords:
acrocentric chromosome; RNA polymerase I; upstream binding factor (UBF); zinc finger; rDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Tseng, H Childrens Hosp Philadelphia, Dept Dermatol, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 19104 USA
Citazione:
H. Tseng et al., "Basonuclin is associated with the ribosomal RNA genes on human keratinocyte mitotic chromosomes", J CELL SCI, 112(18), 1999, pp. 3039-3047

Abstract

Basonuclin is a zinc finger protein mainly expressed in keratinocytes of the basal layer of epidermis and the outer root sheath of hair follicles, Itis also found in abundance in the germ cells of testis and ovary. In cultured keratinocytes, basonuclin is associated with chromatin in all phases ofthe cell cycle, including mitosis. By immunocytochemical methods, we demonstrate here that in mitosis basonuclin is associated with the short arms ofthe acrocentric chromosomes and with other loci on many metaphase chromosomes of human keratinocytes. Using the evolutionarily highly conserved N-terminal pair of zinc fingers in an electrophoresis mobility shift assay, we demonstrate that the DNA target sequences of basonuclin on the acrocentric chromosomes are likely to be within the promoter region of the 45S rRNA genetranscription unit. DNase I footprinting shows that basonuclin zinc fingers interact with the upstream control element of this promoter, which is necessary for the high level of transcription of the rRNA genes. This result suggests that basonuclin may be a tissue-specific transcription factor for the ribosomal RNA genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:42:10