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Titolo:
A novel in vivo assay reveals inhibition of ribosomal nuclear export in Ran-cycle and nucleoporin mutants
Autore:
Hurt, E; Hannus, S; Schmelzl, B; Lau, D; Tollervey, D; Simos, G;
Indirizzi:
Biochem Zentrum Heidelberg, D-69120 Heidelberg, Germany Biochem Zentrum Heidelberg Heidelberg Germany D-69120 eidelberg, Germany Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 3JR Midlothian, Scotland
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 3, volume: 144, anno: 1999,
pagine: 389 - 401
SICI:
0021-9525(19990208)144:3<389:ANIVAR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPORT; PROTEIN IMPORT; SACCHAROMYCES-CEREVISIAE; PORE COMPLEX; NUCLEOCYTOPLASMIC TRANSPORT; BINDING PROTEIN; POLY(A)(+) RNA; YEAST MUTANT; FUNCTIONAL-ANALYSIS; CELL-NUCLEUS;
Keywords:
nuclear pore complex; ribosomal export; L25; GFP; nucleocytoplasmic transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
78
Recensione:
Indirizzi per estratti:
Indirizzo: Hurt, E Biochem Zentrum Heidelberg, Neuenheimer Feld 328, D-69120 Heidelberg, Germany Biochem Zentrum Heidelberg Neuenheimer Feld 328 Heidelberg Germany D-69120
Citazione:
E. Hurt et al., "A novel in vivo assay reveals inhibition of ribosomal nuclear export in Ran-cycle and nucleoporin mutants", J CELL BIOL, 144(3), 1999, pp. 389-401

Abstract

To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently exported into the cytoplasm. In wild-type cells, GFP-labeled ribosomes are only detected by fluorescence in the cytoplasm, However, thermosensitive mal-l (Ran-GAP), prp20-1 (Ran-GEF), and nucleoporin nup49 and nsp1 mutants areimpaired in ribosomal export as revealed by nuclear accumulation of L25-GFP, Furthermore, overexpression of dominant-negative RanGTP (Gsp1-G21V) and the tRNA exportin Los1p inhibits ribosomal export. The pattern of subnuclear accumulation of L25-GFP observed in different mutants is not identical, suggesting that transport can be blocked at different steps. Thus, nuclear export of ribosomes requires the nuclear/cytoplasmic Ran-cycle and distinct nucleoporins. This assay can be used to identify soluble transport factors required for nuclear exit of ribosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:35:02