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Titolo:
Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubdes
Autore:
Oegema, K; Wiese, C; Martin, OC; Milligan, RA; Iwamatsu, A; Mitchison, TJ; Zheng, YX;
Indirizzi:
Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ed, Dept Cell Biol, Boston, MA 02115 USA Carnegie Inst Washington, Dept Embryol, Baltimore, MD 21210 USA Carnegie Inst Washington Baltimore MD USA 21210 , Baltimore, MD 21210 USA Scripps Clin & Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Kirin Brewery Co Ltd, Cent Labs Key Technol, Yokohama, Kanagawa 236, JapanKirin Brewery Co Ltd Yokohama Kanagawa Japan 236 ama, Kanagawa 236, Japan
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 144, anno: 1999,
pagine: 721 - 733
SICI:
0021-9525(19990222)144:4<721:COTRDG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE POLE BODY; SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-NIDULANS; ORGANIZING CENTER; RING COMPLEX; IN-VITRO; CENTROSOME; PROTEIN; SPC98P; CELLS;
Keywords:
centrosome; cytoskeleton; nucleation; GTP; GDP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Oegema, K European Mol Biol Lab, Cell Biol Program, Meyerhofstr 1, D-69117Heidelberg, Germany European Mol Biol Lab Meyerhofstr 1 Heidelberg Germany D-69117
Citazione:
K. Oegema et al., "Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubdes", J CELL BIOL, 144(4), 1999, pp. 721-733

Abstract

gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1-12). Here, we report the purification and characterization of both complexes that we name gamma-tubulin small complex (gamma TuSC; similar to 280,000 D) and Drosophila gamma TuRC (similar to 2,200,000 D). In addition to gamma-tubulin, the gamma TuSC contains Dgrip84 and Dgrip91, two proteins homologous to the Spc97/98p protein family. The gamma TuSC is a structural subunit of the gamma TuRC, a larger complex containing about six additional polypeptides. Like the gamma TuRC isolated from Xenopus egg extracts (Zheng,Y., M.L. Wong, B. Alberts, and T. Mitchison. 1995. Nature. 378:578-583), the Drosophila gamma TuRC can nucleate microtubules in vitro and has an openring structure with a diameter of 25 nm. Cryo-electron microscopy reveals a modular structure with similar to 13 radially arranged structural repeats. The gamma TuSC also nucleates microtubules, but much less efficiently than the gamma TuRC, suggesting that assembly into a larger complex enhances nucleating activity. Analysis of the nucleotide content of the gamma TuSC reveals that gamma-tubulin binds preferentially to GDP over GTP, rendering gamma-tubulin an unusual member of the tubulin superfamily.

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Documento generato il 15/01/21 alle ore 23:00:54