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Titolo:
Motility analysis and energetics of the Siberian sturgeon Acipenser baeriispermatozoa
Autore:
Billard, R; Cosson, J; Fierville, F; Brun, R; Rouault, T; Williot, P;
Indirizzi:
Museum Natl Hist Nat, Lab Ichtyol, F-75231 Paris 05, France Museum Natl Hist Nat Paris France 05 b Ichtyol, F-75231 Paris 05, France CNRS, URA 671, Stn Zool, F-06230 Villefranche Sur Mer, France CNRS Villefranche Sur Mer France F-06230 30 Villefranche Sur Mer, France Cemagref Bordeaux, F-33612 Cestas, France Cemagref Bordeaux Cestas France F-33612 Bordeaux, F-33612 Cestas, France
Titolo Testata:
JOURNAL OF APPLIED ICHTHYOLOGY-ZEITSCHRIFT FUR ANGEWANDTE ICHTHYOLOGIE
fascicolo: 4-5, volume: 15, anno: 1999,
pagine: 199 - 203
SICI:
0175-8659(199909)15:4-5<199:MAAEOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM MOTILITY; CYPRINUS-CARPIO; COMMON CARP; MOVEMENT; FRESH; FISH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Billard, R Museum Natl Hist Nat, Lab Ichtyol, 43 Rue Cuvier, F-75231 Paris05, France Museum Natl Hist Nat 43 Rue Cuvier Paris France 05 05, France
Citazione:
R. Billard et al., "Motility analysis and energetics of the Siberian sturgeon Acipenser baeriispermatozoa", J APP ICHTH, 15(4-5), 1999, pp. 199-203

Abstract

A study of motility was carried out on spermatozoa of the Siberian sturgeon (Acipenser baerii Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10-20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 +/- 1.85 to 4.05 +/- 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs(in vivo survival) do not show significant change in the percentage of motile cells (82 +/- 14 vs 74 +/- 15) nor in the ATP content (6.13 +/- 2.37 vs4.59 +/- 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4 degrees C shows a significant decrease in the percentage of motile cells from 82 +/- 14 to 59 +/- 30 (P<0.01) and in the ATP content from 6.15 +/- 2.4 to 1.95+/- 1.37 nmoles/108 spermatozoa (P=0.001).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:09:41