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Titolo:
Effects of epoxycarotenoids, beta-carotene, and retinoic acid on the differentiation and viability of the leukemia cell line NB4 in vitro
Autore:
Duitsman, PK; Barua, AB; Becker, B; Olson, JA;
Indirizzi:
Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 Biophys & Mol Biol, Ames, IA 50011 USA
Titolo Testata:
INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH
fascicolo: 5, volume: 69, anno: 1999,
pagine: 303 - 308
SICI:
0300-9831(199909)69:5<303:EOEBAR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE PROMYELOCYTIC LEUKEMIA; GAP JUNCTIONAL COMMUNICATION; VITAMIN-A; NEOPLASTIC TRANSFORMATION; PRECURSOR CANTHAXANTHIN; INDUCTION; GROWTH; SOLUBILIZATION; PROLIFERATION; GLUCURONIDE;
Keywords:
epoxycarotenoids; beta-carotene; retinoic acid; 5,6-epoxyretinoic acid; cell differentiation; viability; NB4 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Olson, JA Iowa State Univ, Dept Biochem Biophys & Mol Biol, 3252 Mol Biol Bldg, Ames, IA 50011 USA Iowa State Univ 3252 Mol Biol Bldg Ames IA USA 50011 A 50011 USA
Citazione:
P.K. Duitsman et al., "Effects of epoxycarotenoids, beta-carotene, and retinoic acid on the differentiation and viability of the leukemia cell line NB4 in vitro", INT J VIT N, 69(5), 1999, pp. 303-308

Abstract

Three all-trans epoxides of beta-carotene (beta-Car), namely, 5,6-epoxy-beta-carotene (5,6-EC) 5,8-epoxy-b-carotene (5,8-EC) and 5,6,5',6'-diepoxy-beta-carotene (5,6,5',6'-DEC) were synthesized by treatment of beta-carotene with 3-chloroperoxybenzoic acid, were purified chromatographically, and were characterized. The relative potencies (mean +/- S.D.) of 1 mM compounds in inducing the differentiation of NB4 cells, a cell line that contains the chromosomal transposition t(15;17) characteristic of acute promyelocytic leukemia, after 4 days of incubation were: RA: 1.35 +/- 0.16, 5,6-EC: 0.29 +/- 0.01, 5,8-EC: 0.22 +/- 0.05, 5,6,5',6'-DEC: 0.11 +/- 0.02, beta C: 0.09 +/- 0.01, and the control: 0.06 +/- 0.01. The same order of potencies existed at other concentrations tested and at other incubation times. P values for the differences between the:inducing activities of successive pans of compounds at 1 mu M were: RA vs. 5,6-EC, < 0.001; 5,6-EC vs. 5,8-EC, < 0.01; 5,8-EC vs. 5,6,5',6'-DEC, < 0.01; 5,6,5',6'-DEC vs. beta-Car, < 0.10; beta-Car vs. control, < 0.005. Similar P values were also obtained for studies atother concentrations and at other incubation times. The viable cell mass at 4 days was inversely proportional to the extent of differentiation (r(s) = -1.0). The inducing activities of all compounds were dose-dependent. Thus, the 5,6-monoepoxide of beta-carotene, which has not previously been studied as an inducer, showed higher activity in NB4 cell differentiation than the 5,8-monoepoxide, the 5,6,5',6'-diepoxide, or beta-carotene. Possible explanations of these observations are discussed.

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Documento generato il 28/11/20 alle ore 00:31:45