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Titolo:
Inhibition of neutrophil derived lysosomal enzymes and reactive oxygen species by a novel tetrapeptide
Autore:
Meera, R; Anand, S; Ramesh, CV; Puvanakrishnan, R;
Indirizzi:
Cent Leather Res Inst, Dept Biotechnol, Madras 600020, Tamil Nadu, India Cent Leather Res Inst Madras Tamil Nadu India 600020 0, Tamil Nadu, India
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 9, volume: 48, anno: 1999,
pagine: 479 - 484
SICI:
1023-3830(199909)48:9<479:IONDLE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; FIBRONECTIN FRAGMENTS; CARTILAGE DEGRADATION; RHEUMATOID-ARTHRITIS; PLATELET-AGGREGATION; TISSUE; BINDING; LACTOTRANSFERRIN; MYELOPEROXIDASE; INFLAMMATION;
Keywords:
polymorphonuclear neutrophils; reactive oxygen species; tetrapeptide derivative; lysosomal enzymes and adjuvant induced arthritis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Puvanakrishnan, R Cent Leather Res Inst, Dept Biotechnol, Madras 600020, Tamil Nadu, India Cent Leather Res Inst Madras Tamil Nadu India 600020 ia
Citazione:
R. Meera et al., "Inhibition of neutrophil derived lysosomal enzymes and reactive oxygen species by a novel tetrapeptide", INFLAMM RES, 48(9), 1999, pp. 479-484

Abstract

Objective and Design: The role of a tetrapeptide derivative PEP 1261 {Boc-Lys(Boc)-Arg-Asp-Ser(tBu)-OtBu}, corresponding to residues 39-42 of human lactoferrin, has been tested in vitro in the modulation of neutrophil function. Material and Subjects: The level of non-enzymatic mediators of inflammation such as reactive oxygen species (ROS), enzymatic mediators such as myeloperoxidase (MPO) and lysosomal enzymes have been assessed in the presence orabsence of PEP 1261 in phorbol 12-myristate 13 acetate (PMA) stimulated human neutrophils (n = 6) and also in neutrophils isolated from adjuvant induced arthritic rats (AIA) (n = 4). Treatment: PEP 1261, at a concentration of 0.14 mM, was added to the neutrophil cultures. Statistical Method: The results were analysed by nonparametric statistics using Mann Whitney U test. Results: Addition of PEP 1261 effectively blocked the H2O2 and O-2(.-) release, decreased the levels of MPO levels (p < 0.01) and lysosomal enzymes (p < 0.05) as compared to PMA stimulated human neutrophils. PEP 1261 was also observed to inhibit the levels of H2O2, O-2(.-), MPO and lysosomal enzymes (p < 0.05) as compared to PMA stimulated control rat neutrophils and neutrophils from arthritic rats. Conclusions: The results of this study indicate that PEP 1261 could serve as an excellent antiinflammatory agent.

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Documento generato il 27/09/20 alle ore 22:49:16