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Titolo:
Identification and immunological characterization of a novel 40-kDa protein linked to CD98 antigen
Autore:
Matsumoto, Y; Satoh-Ueno, K; Yoshimura, A; Hashimoto, Y; Enomoto, T; Masuko, T;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Mol Cell Biol Lab, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan Sasaki Inst, Chiyoda Ku, Tokyo 1010062, Japan Sasaki Inst Tokyo Japan 1010062 i Inst, Chiyoda Ku, Tokyo 1010062, Japan
Titolo Testata:
CELL STRUCTURE AND FUNCTION
fascicolo: 4, volume: 24, anno: 1999,
pagine: 217 - 226
SICI:
0386-7196(199908)24:4<217:IAICOA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; MURINE MONOCLONAL-ANTIBODIES; XENOPUS-LAEVIS OOCYTES; SURFACE-ANTIGEN; HEAVY-CHAIN; ACTIVATED LYMPHOCYTES; INHIBIT ACTIVATION; CANCER-CELLS; EXPRESSION; 4F2;
Keywords:
CD98; GP125 heavy chain (HC); GP125 light chain (LC); monoclonal antibody (mAb); amino-acid transporter; malignant transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Masuko, T Tohoku Univ, Grad Sch Pharmaceut Sci, Mol Cell Biol Lab, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 , Miyagi 9808578, Japan
Citazione:
Y. Matsumoto et al., "Identification and immunological characterization of a novel 40-kDa protein linked to CD98 antigen", CELL STRUCT, 24(4), 1999, pp. 217-226

Abstract

Monoclonal antibodies (mAbs) were obtained from hybridoma clones established by cell fusion between mouse myeloma cells and spleen cells from a mouseimmunized against an affinity-purified 40kDa component of rat 125-kDa glycoprotein (GP125). Two mAbs designated as 3F2 and 6B4 detected a 40-kDa and a 125-kDa band under reducing and nonreducing conditions, respectively, in extracts prepared from rat, mouse and human tumor cells. Association of the40-kDa protein with CD98 was revealed by sandwich-type enzyme-linked immunosorbent assay. The two mAbs were strongly reactive with various tumor cells and activated lymphocytes, but were only weakly reactive with resting lymphocytes. Confocal microscopy indicated colocalization of CD98 and the 40-kDa protein defined with 3F2 and 6B4 at the cell surface and perinuclear regions. On immunohistochemical analysis of frozen sections of rat tongue, theanti-rat CD98 mAb B3 selectively stained the basal layer and 3F2 stained the upper epithelial part in addition to the basal layer, indicating the existence of CD98-unlinked 40-kDa protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:06:35