Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning
Autore:
Horst, CJ; Fishkind, DJ; Pazour, GJ; Witman, GB;
Indirizzi:
Worcester Fdn Biomed Res, Shrewsbury, MA USA Worcester Fdn Biomed Res Shrewsbury MA USA iomed Res, Shrewsbury, MA USA
Titolo Testata:
CELL MOTILITY AND THE CYTOSKELETON
fascicolo: 2, volume: 44, anno: 1999,
pagine: 143 - 154
SICI:
0886-1544(199910)44:2<143:AIMOCR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIVIDING CULTURED-CELLS; BASAL BODIES; FLAGELLA; DYNEIN; GENE; APPARATUS; ENCODES; TUBULIN; PROTEIN; ORGANIZATION;
Keywords:
Chlamydomonas; cytoskeleton; microtubules; mitosis; cell shape; MTOC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Horst, CJ Carroll Coll, Dept Biol, 100 N East Ave, Waukesha, WI 53186 USA Carroll Coll 100 N East Ave Waukesha WI USA 53186 , WI 53186 USA
Citazione:
C.J. Horst et al., "An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning", CELL MOTIL, 44(2), 1999, pp. 143-154

Abstract

cmul-1 is a new mutation, of Chlamydomonas reinhardtii that causes a change in cell shape due to an alteration of cytoplasmic microtubule organization. cmul mutant cells were first identified based on their altered cell shape. Unlike wild-type cells, which are ellipsoid, cmul cells tend to be either round or egg-shaped with the flagella extending from the narrow end of the cell. Electron microscopic comparison of mutant and wild-type cells indicated that microtubule distribution was altered in the mutant cells. Immunofluorescence microscopy using anti-P-tubulin antibodies revealed that, in wild-type cells, microtubules arise from the anterior end of the cell in the region of the basal bodies, pass posteriorly subjacent to the plasma membrane, and terminate near the posterior end of the cell. In mutant cells, the microtubules also arise from the basal body region but then become disarrayed. They frequently curl back anteriorly or wrap around the equator of the cell; some microtubules also extend completely to the posterior end of the cell, then turn back toward the anterior end. No changes in the basal body region were detected by electron microscopy. Some cmul cells had multiple nuclei or an aberrant number of flagella, both of which may be due to defects in cell division, a process dependent upon microtubules. Thus, cmul-1, which was generated by insertional mutagenesis and is tagged, appears to encode a protein that plays an essential role in the spatial organization of cytoplasmic microtubules involved in both interphase and mitotic functions. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 21:42:03