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Titolo:
Homing of human cells in the fetal sheep model: Modulation by antibodies activating or inhibiting very late activation antigen-4-dependent function
Autore:
Zanjani, ED; Flake, AW; Almeida-Porada, G; Tran, N; Papayannopoulou, T;
Indirizzi:
Univ Washington, Div Hematol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Div Hematol, Seattle, WA 98195 USA VA Med Ctr, Reno, NV USA VA Med Ctr Reno NV USAVA Med Ctr, Reno, NV USA Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA
Titolo Testata:
BLOOD
fascicolo: 7, volume: 94, anno: 1999,
pagine: 2515 - 2522
SICI:
0006-4971(19991001)94:7<2515:HOHCIT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; TRANSPLANTED MARROW-CELLS; TERM REPOPULATING ABILITY; COLONY-FORMING CELLS; BONE-MARROW; IN-VIVO; ADHESION MOLECULE-1; IRRADIATED MICE; ENGRAFTMENT; EFFICIENCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Papayannopoulou, T Univ Washington, Div Hematol, Box 357710, Seattle, WA 98195 USA Univ Washington Box 357710 Seattle WA USA 98195 195 USA
Citazione:
E.D. Zanjani et al., "Homing of human cells in the fetal sheep model: Modulation by antibodies activating or inhibiting very late activation antigen-4-dependent function", BLOOD, 94(7), 1999, pp. 2515-2522

Abstract

The mechanisms by which intravenously (IV)-administered homing, we also pretreated human donor cells with an hematopoietic cells home to the bone marrow (BM) are activating antibody to pi integrins. This treatment resulted poorly defined. Although insightful information has been obtained in mice, our knowledge about homing of human cells is very limited. In the present study, we investigated the importance of very late activation antigen (VLA)-4in the early phases of lodgment of human CD34(+) progenitors into the sheep hematopoietic compartment after in utero transplantation, We have found that preincubation of donor cells with anti-VLA-4 blocking antibodies resulted in a profound reduction of human cell lodgment in the fetal BM at 24 and48 hours after transplantation, with a corresponding increase of human cells in the peripheral circulation. Furthermore, IV infusion of the anti-VLA-4 antibody at later times (posttransplantation days 21 to 24) resulted in redistribution or mobilization of human progenitors from the BM to the peripheral blood. In an attempt to positively modulate in increased lodgment of donor cells in the fetal liver, presumably for hemodynamic reasons, at the expense of the BM, Given previous involvement of the VLA-4/vascular cell adhesion molecule (VCAM)-1 adhesion pathway in homing and mobilization in themurine system, our present data suggest that cross-reacting ligands (likely VCAM-1) for human VLA-4 exist in sheep BM, thereby implicating conservation of molecular mechanisms of homing and mobilization across disparate species barriers. Thus, information from xenogeneic models of human hematopoiesis and specifically, the human/sheep model of in utero transplantation, mayprovide valuable insights into human hematopoietic transplantation biology. (C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:59:37