Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation
Autore:
Anderson, KL; Smith, KA; Perkin, H; Hermanson, G; Anderson, CG; Jolly, DJ; Maki, RA; Torbett, BE;
Indirizzi:
Scripps Clin & Res Inst, Dept Immunol, La Jolla, CA 92037 USA Scripps Clin& Res Inst La Jolla CA USA 92037 nol, La Jolla, CA 92037 USA Burnham Inst, La Jolla, CA 92037 USA Burnham Inst La Jolla CA USA 92037Burnham Inst, La Jolla, CA 92037 USA Vical Inc, San Diego, CA USA Vical Inc San Diego CA USAVical Inc, San Diego, CA USA Chiron Technol Ctr Gene Therapy, San Diego, CA USA Chiron Technol Ctr GeneTherapy San Diego CA USA rapy, San Diego, CA USA
Titolo Testata:
BLOOD
fascicolo: 7, volume: 94, anno: 1999,
pagine: 2310 - 2318
SICI:
0006-4971(19991001)94:7<2310:PATGAM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-ALPHA; RETROVIRAL VECTORS; C/EBP-ALPHA; DEFICIENT MICE; GENE-TRANSFER; EXPRESSION; PROGENITORS; MOUSE; GRANULOPOIESIS; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Torbett, BE Scripps Clin & Res Inst, Dept Immunol, IMM-7,10550 N Torrey Pines Rd, La Jolla, CA 92037 USA Scripps Clin & Res Inst IMM-7,10550 N TorreyPines Rd La Jolla CA USA 92037
Citazione:
K.L. Anderson et al., "PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation", BLOOD, 94(7), 1999, pp. 2310-2318

Abstract

PU.1 is a hematopoietic cell-specific ets family transcription factor. Gene disruption of PU.1 results in a cell autonomous defect in hematopoietic progenitor cells that manifests as abnormal myeloid and B-lymphoid development. Of the myeloid lineages, no mature macrophages develop, and the neutrophils that develop are aberrantly and incompletely matured. One of the documented abnormalities of PU.1 null (deficient) hematopoietic cells is a failure to express receptors for granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM)-CSF, and M-CSF, To elucidate the roles of the myeloid growth factor receptors in myeloid cell differentiation, and to distinguish their role from that of PU.1, we have restored expression of the G-and WI-CSF receptors in PU.1-deficient cells using retroviral vectors. We have similarly expressed PU.1 in these cells. Whereas expression of growth factor receptors merely allows a PU.1-deficient cell line to survive and grow in the relevant growth factor, expression of PU.1 enables the development of F4/80(+), Mac-1(+)/CD11b(+) macrophages, expression of gp91(phox) and generation of superoxide, and expression of secondary granule genes for neutrophil collagenase and gelatinase, These studies reinforce the idea that availability of PU.1 is crucial for normal myeloid development and clarify some of the molecular events in developing neutrophils and macrophages that are critically dependent on PU.1. (C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:46:24