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Titolo:
Fermentations with new recombinant organisms
Autore:
Bothast, RJ; Nichols, NN; Dien, BS;
Indirizzi:
ARS, Fermentat Biochem Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA ARS Peoria IL USA 61604 Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 15, anno: 1999,
pagine: 867 - 875
SICI:
8756-7938(199909/10)15:5<867:FWNRO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZYMOMONAS-MOBILIS GENES; XYLOSE ISOMERASE GENE; ESCHERICHIA-COLI-B; LACTATE DEHYDROGENASE GENE; ETHANOL-PRODUCTION; KLEBSIELLA-OXYTOCA; CORN FIBER; SACCHAROMYCES-CEREVISIAE; PACHYSOLEN-TANNOPHILUS; LACTOBACILLUS-PLANTARUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Bothast, RJ ARS, Fermentat Biochem Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA ARS 1815 N Univ St Peoria IL USA 61604 t, Peoria, IL 61604 USA
Citazione:
R.J. Bothast et al., "Fermentations with new recombinant organisms", BIOTECH PR, 15(5), 1999, pp. 867-875

Abstract

United States fuel ethanol production in 1998 exceeded the record production of 1.4 billion gallons set in 1995. Most of this ethanol was produced from over 550 million bushels of corn. Expanding fuel ethanol production willrequire developing lower-cost feedstocks, and only lignocellulosic feedstocks are available in sufficient quantities to substitute for corn starch. Major technical hurdles to converting lignocellulose to ethanol include the lack of low-cost efficient enzymes for saccharification of biomass to fermentable sugars and the development of microorganisms for the fermentation ofthese mixed sugars. To date, the most successful research approaches to develop navel biocatalysts that will efficiently ferment mixed sugar syrups include isolation of novel yeasts that ferment xylose, genetic engineering of Escherichia coli and other gram negative bacteria for ethanol production,and genetic engineering of Saccharoymces cerevisiae and Zymomonas mobilis for pentose utilization. We have evaluated the fermentation of corn fiber hydrolyzates by the various strains developed. E. coli K011, E. coli SL40, E. coli FBR3, Zymomonas CP4 (pZB5), and Saccharomyces 1400 (pLNH32) fermented corn fiber hydrolyzates to ethanol in the range of 21-34 g/L with yields ranging from 0.41 to 0.50 g of ethanol per gram of sugar consumed. Progresswith new recombinant microorganisms has been rapid and will continue with the eventual development of organisms suitable for commercial ethanol production. Each research approach holds considerable promise, with the possibility existing that different "industrially hardened" strains may find separate applications in the fermentation of specific feedstocks.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:44:00