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Titolo:
Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus
Autore:
Jin, SJ; Gu, XX; Rhim, JS; Lim, DJ;
Indirizzi:
House Ear Inst, Dept Cell & Mol Biol, Los Angeles, CA 90057 USA House Ear Inst Los Angeles CA USA 90057 l Biol, Los Angeles, CA 90057 USA NIDOCD, Lab Cellular Biol, NIH, Rockville, MD USA NIDOCD Rockville MD USA IDOCD, Lab Cellular Biol, NIH, Rockville, MD USA NCI, Lab Biochem Physiol, NIH, Frederick, MD 21701 USA NCI Frederick MD USA 21701 Biochem Physiol, NIH, Frederick, MD 21701 USA
Titolo Testata:
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
fascicolo: 10, volume: 108, anno: 1999,
pagine: 934 - 943
SICI:
0003-4894(199910)108:10<934:IOCMEE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONTYPABLE HAEMOPHILUS-INFLUENZAE; EXPERIMENTAL OTITIS-MEDIA; MONOCLONAL-ANTIBODIES; CYTOKERATIN PATTERNS; EXPRESSION; CULTURE; TISSUES; TRANSFORMATION; CHOLESTEATOMA; PATHOGENESIS;
Keywords:
adenovirus 12-simian virus 40; cell culture; cell line; chinchilla middle ear epithelial cell; immortalization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lim, DJ House Ear Inst, Dept Cell & Mol Biol, 2100 W 3rd St, Los Angeles, CA 90057USA House Ear Inst 2100 W 3rd St Los Angeles CA USA 90057 CA 90057USA
Citazione:
S.J. Jin et al., "Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus", ANN OTOL RH, 108(10), 1999, pp. 934-943

Abstract

In order to study the cellular and molecular mechanisms of the pathogenesis of otitis media, a chinchilla middle ear epithelial cell tine (CMEE-I) with differentiated cell characteristics was established by infection of a primary culture with the adenovirus 12-simian virus 40 (Ad12-SV40) hybrid. This cell line has been in continuous culture for 42 passages, whereas the parent cells underwent senescence and died at the 8th passage. The cell line also retains epithelial morphology and expresses cytokeratin polypeptides 4, 7, and 18, characteristic markers for epithelia. In Western blots of cellproteins, bands at 94 and 53 kd were labeled after binding antibodies against SV40 large T antigen and p53, respectively. Karyotype analysis showed that the cell line is derived from chinchilla epithelial cells. These findings confirm that the cell line is a chinchilla epithelial cell immortalized by the hybrid virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:09:36