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Titolo:
Evaluation of purification procedures for DNA extracted from organic rich samples: Interference with humic substances
Autore:
Harry, M; Gambier, B; Bourezgui, Y; Garnier-Sillam, E;
Indirizzi:
Univ Paris 12 Val Marne, Lab Biol Sols & Eaux, F-94000 Creteil, France Univ Paris 12 Val Marne Creteil France F-94000 , F-94000 Creteil, France
Titolo Testata:
ANALUSIS
fascicolo: 5, volume: 27, anno: 1999,
pagine: 439 - 442
SICI:
0365-4877(199906)27:5<439:EOPPFD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MICROBIAL DNA; SOIL; SEDIMENTS; MOUNDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Harry, M Univ Paris 12 Val Marne, Lab Biol Sols & Eaux, Ave Gen Gaulle, F-94000 Creteil, France Univ Paris 12 Val Marne Ave Gen Gaulle Creteil France F-94000 ce
Citazione:
M. Harry et al., "Evaluation of purification procedures for DNA extracted from organic rich samples: Interference with humic substances", ANALUSIS, 27(5), 1999, pp. 439-442

Abstract

A limitation of the use of the Polymerase Chain Reaction (PCR) for soil DNA analysis is the contamination by humic substances. Numerous studies have been devoted to the elaboration of an effective purification method but none appears universal. During our investigations of soil bacterial changes induced by soil-feeding termites, we found that humic acid content usually impede DNA purification. Indeed, humic acids and nucleic acids share similar physicochemical properties. Here, we tested eight purification procedures including electrophoretical and chromatographical methods. The results show for DNA extracted from humic rich samples, e.g. termite mounds, that only the combination of two methods gave a DNA sufficiently pure to perform successful amplifications.

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Documento generato il 02/10/20 alle ore 01:32:37