Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast
Autore:
Chelstowska, A; Liu, ZC; Jia, YK; Amberg, D; Butow, RA;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 d Ctr, Dept Mol Biol, Dallas, TX 75235 USA SUNY Hlth Sci Ctr, Dept Biochem & Mol Biol, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr Syracuse NY USA 13210 Mol Biol, Syracuse, NY 13210 USA
Titolo Testata:
YEAST
fascicolo: 13, volume: 15, anno: 1999,
pagine: 1377 - 1391
SICI:
0749-503X(19990930)15:13<1377:SBMATN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCODING PEROXISOMAL PROTEINS; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; BINDING DOMAIN; ACTIN; COMMUNICATION; OXIDOREDUCTASE; MEMBRANE; RTG2;
Keywords:
yeast; mitochondria; retrograde regulation; D-lactate dehydrogenase activities;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Butow, RA Univ Texas, SW Med Ctr, Dept Mol Biol, 5323 Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 X 75235 USA
Citazione:
A. Chelstowska et al., "Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast", YEAST, 15(13), 1999, pp. 1377-1391

Abstract

We have adapted a LacZ promoter trap screen developed by Burns et al. (1994) to search for genes whose expression is dependent on Rtg2p, a protein with an N-terminal hsp70/actin/sugar kinase ATP binding domain. Rtg2p acts upstream of the basic helix-loop-helix/leucine zipper transcription factors, Rtg1p and Rtg3p. All three proteins are known to be required for the expression of the CIT2 gene, which encodes a peroxisomal isoform of citrate synthase whose expression is also dependent on the functional state of mitochondria. Using this screen, we have identified a previously uncharacterized gene, YEL071w, predicted to encode a protein of 496 amino acids that shares 80% homology and 60% sequence identity with actin interacting protein 2, encoded by the AIP2 gene; both proteins also share sequence similarity to a D-lactate dehydrogenase encoded by the DLD1 gene. Expression of YEL071w is dependent on the functional state of mitochondria and on all three of the Rtg proteins, whereas AIP2 expression is independent of the Rtg proteins and the functional state of mitochondria. Like CIT2, the 5' flanking region of YEL071w contains two R box binding sites for the Rtg1p/Rtg3p heterodimeric transcription complex. Both R boxes are necessary for full YEL071w expression. We show that YEL071w and AIP2 encode proteins with D-lactate dehydrogenase activity, the former located in the cytoplasm and the latter in the mitochondrial matrix. Our data thus provide gene assignments for two previously unrecognized D-lactate dehydrogenase activities in yeast. Copyright (C) 1999 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:37:27