Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Organotypic culture of neuroepithelium attached to olfactory bulb from adult mouse as a tool to study neuronal regeneration after ZnSO4 neuroepithelial trauma
Autore:
Michel, V; Monnier, Z; Cvetkovic, V; Math, F;
Indirizzi:
Neurosci Lab, EA 481, F-25000 Besancon, France Neurosci Lab Besancon France F-25000 b, EA 481, F-25000 Besancon, France Fac Med, Lab Neuroendocrinol Morphofonctionnelle, CNRS, UPRESA 6025, F-25000 Besancon, France Fac Med Besancon France F-25000 S, UPRESA 6025, F-25000 Besancon, France
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 3, volume: 271, anno: 1999,
pagine: 195 - 198
SICI:
0304-3940(19990827)271:3<195:OCONAT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR NEURONS; EPITHELIUM; DEGENERATION; MUCOSA; GROWTH; MICE;
Keywords:
organotypic culture; adult mouse; olfactory system; neuronal regeneration; ZnSO4 neuroepithelial trauma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Michel, V Neurosci Lab, EA 481, Pl Leclere, F-25000 Besancon, France Neurosci Lab Pl Leclere Besancon France F-25000 esancon, France
Citazione:
V. Michel et al., "Organotypic culture of neuroepithelium attached to olfactory bulb from adult mouse as a tool to study neuronal regeneration after ZnSO4 neuroepithelial trauma", NEUROSCI L, 271(3), 1999, pp. 195-198

Abstract

Chemical destruction of the olfactory mucosa leads to a neuronal regeneration. A new organotypic culture model is perfected to improve the regenerating processes study. Explants of neuroepithelium attached to olfactory bulbswere removed from adult mice and cultured, 12 h after ZnSO4 intranasal application. After 3 days in culture, explants showed a necrosis in the olfactory epithelium and a thinning of the olfactory bulb nervous layer. From thefifth day of culture, and mostly the tenth, new cells showed positive immunoreactivity with the olfactory marker protein (OMP), meaning they were regenerating olfactory neurons. Simultaneously, OMP immunoreactivity increasedin the nervous and glomerular layers of the olfactory bulb, indicating epithelio-bulbar reconnection. This organotypic culture model could allow further investigations on the regenerating process kinetic. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:16:02