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Titolo:
Quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other Gram-negative bacteria
Autore:
Holden, MTG; Chhabra, SR; de Nys, R; Stead, P; Bainton, NJ; Hill, PJ; Manefield, M; Kumar, N; Labatte, M; England, D; Rice, S; Givskov, M; Salmond, GPC; Stewart, GSAB; Bycroft, BW; Kjelleberg, SA; Williams, P;
Indirizzi:
Univ Nottingham, Sch Pharmaceut Sci, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England Univ New S Wales, Sch Biol Sci, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 i, Sydney, NSW 2052, Australia Univ New S Wales, Sch Microbiol & Immunol, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 l, Sydney, NSW 2052, Australia Univ New S Wales, Sch Chem, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 m, Sydney, NSW 2052, Australia Tech Univ Denmark, Dept Microbiol, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 robiol, DK-2800 Lyngby, Denmark Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 6, volume: 33, anno: 1999,
pagine: 1254 - 1266
SICI:
0950-382X(199909)33:6<1254:QCTIAC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTONE SIGNAL MOLECULES; ACID METHYL-ESTER; HOST-SPECIFIC PHYTOTOXIN; FISCHERI LUXR PROTEIN; VIBRIO-FISCHERI; XANTHOMONAS-CAMPESTRIS; VIRULENCE DETERMINANTS; SERRATIA-LIQUEFACIENS; ERWINIA-CAROTOVORA; N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
79
Recensione:
Indirizzi per estratti:
Indirizzo: Williams, P Univ Nottingham, Sch Pharmaceut Sci, Univ Pk, Nottingham NG7 2RD, England Univ Nottingham Univ Pk Nottingham England NG7 2RD D, England
Citazione:
M.T.G. Holden et al., "Quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other Gram-negative bacteria", MOL MICROB, 33(6), 1999, pp. 1254-1266

Abstract

In cell-free Pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an N-acylhomoserine lactone (AHL) biosensor, Mass spectrometry and NMR spectroscopy revealed that these compounds were not AHLs but the dike-topiperazines (DKPs), cyclo(Delta Ala-L-Val) and cyclo(L-Pro-L-Tyr) respectively. These compounds were also found in cell-free supernatants from Proteus mirabilis, Citrobacter freundii and Enterobacter agglomerans [cyclo(Delta Ala-L-Val) only]. Although both DKPs were absentfrom Pseudomonas fluorescens and Pseudomonas alcaligenes, we isolated, from both pseudomonads, a third DKP, which was chemically characterized as cyclo(L-Phe-L-Pro), Dose-response curves using a LuxR-based AHL biosensor indicated that cyclo(Delta Ala-L-Val), cyclo(L-Pro-L-Tyr) and cyclo(L-Phe-L-Pro) activate the biosensor in a concentration-dependent manner, albeit at much higher concentrations than the natural activator N-(3-oxohexanoyi)-L-homoserine lactone (3-oxo-C6-HSL), Competition studies showed that cyclo(Delta Ala-L-Val), cyclo(L-Pro-L-Tyr) and cyclo(L-Phe-L-Pro) antagonize the 3-oxo-C6-HSL-mediated induction of bioluminescence, suggesting that these DKPs may compete for the same LuxR-binding site. Similarly, DKPs were found to be capable of activating or antagonizing other LuxR-based quorum-sensing systems, such as the N-butanoylhomoserine lactone-dependent swarming motility ofSerratia liquefaciens. Although the physiological role of these DKPs has yet to be established, their activity suggests the existence of cross talk among bacterial signalling systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:58:21