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Titolo:
p120 acts as a specific coactivator for 9-cis-retinoic acid receptor (RXR)on peroxisome proliferator-activated receptor-gamma/RXR heterodimers
Autore:
Monden, T; Kishi, M; Hosoya, T; Satoh, T; Wondisford, FE; Hollenberg, AN; Yamada, M; Mori, M;
Indirizzi:
Gunma Univ, Sch Med, Dept Internal Med 1, Maebashi, Gumma 3718511, Japan Gunma Univ Maebashi Gumma Japan 3718511 1, Maebashi, Gumma 3718511, Japan Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 d, Boston, MA 02215 USA Harvard Univ, Sch Med, Boston, MA 02215 USA Harvard Univ Boston MA USA 02215 vard Univ, Sch Med, Boston, MA 02215 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 10, volume: 13, anno: 1999,
pagine: 1695 - 1703
SICI:
0888-8809(199910)13:10<1695:PAAASC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-ALPHA; PPAR-GAMMA; THYROID-HORMONE; RETINOIC ACID; HISTONE ACETYLTRANSFERASE; DIFFERENTIAL EXPRESSION; ALCOHOL-DEHYDROGENASE; TISSUE DISTRIBUTION; NUCLEAR RECEPTORS; GENE-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Monden, T Gunma Univ, Sch Med, Dept Internal Med 1, 3-39-15 Showa Machi, Maebashi, Gumma 3718511, Japan Gunma Univ 3-39-15 Showa Machi Maebashi GummaJapan 3718511 apan
Citazione:
T. Monden et al., "p120 acts as a specific coactivator for 9-cis-retinoic acid receptor (RXR)on peroxisome proliferator-activated receptor-gamma/RXR heterodimers", MOL ENDOCR, 13(10), 1999, pp. 1695-1703

Abstract

p120 was originally isolated as a novel nuclear coactivator for thyroid hormone receptor. In this study, we characterized its interaction and transactivation of peroxisome proliferator-activated receptor-gamma (PPAR gamma) and 9-cis-retinoic acid receptor (RXR) heterodimers. Transient transfection study revealed that p120 enhanced the transcriptional activation of PPAR gamma/RXR induced by PPAR gamma- or RXR-specific ligands. In the glutathione-S-transferase pull-down assay, while steroid receptor co-activator-1 showedapparent interactions with both RXR and PPAR gamma, p120 bound only to RXRin a 9-cis-retinoic acid (RA)-dependent manner and also did not bind to PPAR gamma even in the presence of thiazolidinediones, The yeast two-hybrid analysis showed no interaction of p120 with FFAR gamma under any conditions,and electophoretic mobility shift assay showed apparent DNA-PPAR gamma/RXR/p120 complex formation only in the presence of 9-cis-RA. Furthermore, the yeast three-hybrid assay clearly revealed a significant interaction betweenp120 and PPAR gamma via RXR of PPAR gamma/RXR heterodimer only in the presence of 9-cis-RA, These findings indicate that p120 acts as a specific coactivator for the RXR of PPAR gamma/RXR heterodimer in a 9-cis-RA-dependent manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:37:33