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Titolo:
Differential regulation of cytokine expression after allergen exposure of sensitized rats by cyclosporin A and corticosteroids: Relationship to bronchial hyperresponsiveness
Autore:
Huang, TJ; Newton, R; Haddad, EB; Chung, KF;
Indirizzi:
Natl Heart & Lung Inst, Dept Thorac Med, Imperial Coll, London SW3 6LY, England Natl Heart & Lung Inst London England SW3 6LY l, London SW3 6LY, England Chang Gung Mem Hosp, Keelung Branch, Dept Thorac Med, Keelung, Taiwan Chang Gung Mem Hosp Keelung Taiwan ch, Dept Thorac Med, Keelung, Taiwan
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 3, volume: 104, anno: 1999,
parte:, 1
pagine: 644 - 652
SICI:
0091-6749(199909)104:3<644:DROCEA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN-NORWAY RATS; LATE ASTHMATIC RESPONSE; INTERFERON-GAMMA; T-CELLS; MESSENGER-RNA; AIRWAY HYPERRESPONSIVENESS; ATOPIC ASTHMA; GUINEA-PIGS; ANTIGEN; INTERLEUKIN-2;
Keywords:
cyclosporin A; dexamethasone; bronchial hyperresponsiveness; IL-4; IL-5; IL-10; IFN-gamma; neutrophil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, KF Natl Heart & Lung Inst, Dept Thorac Med, Imperial Coll, Dovehouse St, London SW3 6LY, England Natl Heart & Lung Inst Dovehouse St London England SW3 6LY land
Citazione:
T.J. Huang et al., "Differential regulation of cytokine expression after allergen exposure of sensitized rats by cyclosporin A and corticosteroids: Relationship to bronchial hyperresponsiveness", J ALLERG CL, 104(3), 1999, pp. 644-652

Abstract

Background: Cyclosporin A and dexamethasone exhibit different effects on allergic airway eosinophilia and bronchial hyperresponsiveness (BHR). Objective: We determined whether these mere related to alteration of cytokine expression, particularly with regard to the profile of Th1- versus Th2-derived cytokines,Methods: Brown-Norway rats sensitized with ovalbumin were administered cyclosporine or dexamethasone before ovalbumin aerosol challenge. Bronchial responsiveness was measured 18 to 24 hours after aerosol exposure. Airway cellular influx was determined by bronchoalveolar lavage and tissue immunohistochemistry, The expression of Th1 and Th2 cytokine messenger RNA (mRNA) wasanalyzed by reverse transcriptase-PCR,Results: Ovalbumin exposure induced significant BHR, with increases in eosinophils, lymphocytes, and neutrophils in bronchoalveolar lavage fluid and an increase in eosinophils, CD2(+) and CD8(+), but not CD4(+) T cells, in the airway submucosa, IL-2, IFN-gamma, IL-4, and IL-5 mRNA expression in thelungs of sensitized ovalbumin-exposed rats was increased (P < .05) compared with controls, Cyclosporin A had no significant effect on BHR and neutrophil accumulation but reduced the number of bronchoalveolar lavage eosinophils (P < .002), airway submucosal eosinophils, and CD4(+) and CD8(+) T cells(P < .02), It also suppressed the induced mRNA expression of IL-2, IL-4, IL-5, and IFN-gamma, By contrast, the inflammatory cell influx and mRNA expression for IL-2, IL-4, and IL-5, as well as BHR, were suppressed by dexamethasone, However, an increase in IL-10 and IFN-gamma mRNA expression was found. Conclusion: The differential activities of cyclosporin A and dexamethasoneon inflammatory cell influx, particularly neutrophils, or cytokine expression such as IL-10 and IFN-gamma may underlie their contrasting effects on BHR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:35:13