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Titolo:
A STEREOLOGICAL METHOD FOR ESTIMATING THE TOTAL LENGTH AND SIZE OF MYELIN FIBERS IN HUMAN BRAIN WHITE-MATTER
Autore:
TANG Y; NYENGAARD JR;
Indirizzi:
AARHUS UNIV,STEREOL RES LAB,INST PATHOL,BARTHOLIN BLDG DK-8000 AARHUSDENMARK AARHUS UNIV,INST EXPT CLIN RES,INTERNAL MED CLIN 2 DK-8000 AARHUS DENMARK N SICHUAN MED COLL,DEPT ANAT NANCHONG SICHUAN PEOPLES R CHINA
Titolo Testata:
Journal of neuroscience methods
fascicolo: 2, volume: 73, anno: 1997,
pagine: 193 - 200
SICI:
0165-0270(1997)73:2<193:ASMFET>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MR; SECTIONS; VOLUME; NUMBER; AIDS; LEUKODYSTROPHY; ENCEPHALITIS; HIPPOCAMPUS; ATROPHY; NEURONS;
Keywords:
LENGTH; VOLUME; MYELIN FIBERS; WHITE MATTER; HUMAN BRAIN; STEREOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Y. Tang e J.R. Nyengaard, "A STEREOLOGICAL METHOD FOR ESTIMATING THE TOTAL LENGTH AND SIZE OF MYELIN FIBERS IN HUMAN BRAIN WHITE-MATTER", Journal of neuroscience methods, 73(2), 1997, pp. 193-200

Abstract

A practically unbiased stereological method to obtain estimates of the volume and total length of nerve fibers in brain white matter is described. The sampling scheme is designed so that the majority of brain white matter is left intact, thus providing the possibility for resampling and further analysis. Uniform sampling of one complete hemispherical white matter is performed. The volume fraction of nerve fibers in white matter is estimated by point counting. The total length of nervefibers was estimated from the product of the volume of white matter, obtained with the Cavalieri principle: and the fiber length density, obtained from the isotropic, uniform random sections which were ensuredby the isector. The size of nerve fibers was derived by measuring theprofile diameter perpendicular to its longest axis. The influence of the postmortem fixation delay on nerve fiber parameters was investigated in one dog and one pig. The criteria for identification of nerve fiber profiles at light microscopy were evaluated using electron microscopy. (C) 1997 Elsevier Science B.V.

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Documento generato il 01/12/20 alle ore 15:41:21