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Titolo:
Intracellular pH regulation of rainbow trout and carp thrombocytes
Autore:
Nikinmaa, M; Bogdanova, A; Virkki, LV;
Indirizzi:
Univ Turku, Dept Biol, FIN-20014 Turku, Finland Univ Turku Turku FinlandFIN-20014 , Dept Biol, FIN-20014 Turku, Finland Univ Helsinki, Dept Biosci, Div Anim Physiol, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ol, FIN-00014 Helsinki, Finland
Titolo Testata:
FISH PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 3, volume: 21, anno: 1999,
pagine: 269 - 275
SICI:
0920-1742(199910)21:3<269:IPRORT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LAMPREY LAMPETRA-FLUVIATILIS; NA+/H+ EXCHANGE; HUMAN-PLATELETS; ONCORHYNCHUS-MYKISS; CYTOPLASMIC PH; NA/H EXCHANGE; CYCLIC-AMP; RED-CELLS; ERYTHROCYTES; INHIBITION;
Keywords:
acid-base regulation; catecholamines; fish; sodium-dependent bicarbonate transport; sodium/proton exchange;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Nikinmaa, M Univ Turku, Dept Biol, FIN-20014 Turku, Finland Univ Turku Turku Finland FIN-20014 FIN-20014 Turku, Finland
Citazione:
M. Nikinmaa et al., "Intracellular pH regulation of rainbow trout and carp thrombocytes", FISH PHYS B, 21(3), 1999, pp. 269-275

Abstract

The pH regulation of rainbow trout and carp thrombocytes was investigated using the pH-sensitive fluorescent dye BCECF and a PTI Imagescan image analysis system. The cells were experimentally acidified either using the ammonium chloride prepulse method or perfusing the cells with a saline containing propionate ion. The recovery from the acidification was followed. By using ion substitutions (N-methyl-D-glucamine for sodium; TRIS vs carbon dioxide/bicarbonate buffer) and transport inhibitors (amiloride to inhibit sodium/proton exchange and DIDS to inhibit chloride and bicarbonate dependent acid extrusion mechanisms) the transport pathways were identified. The pH regulatory mechanisms of thrombocytes in both species were similar. The pH recovery after experimental acidification was fully inhibited by removing sodium from the medium. The sodium-dependent acid extrusion had both amiloride-sensitive and DIDS-sensitive components, indicating that both sodium/proton exchange and sodium-dependent bicarbonate transport were involved. The roleof sodium-dependent bicarbonate movements in pH regulation was ascertainedby showing that pH recovery occurred faster at a high than at a low bicarbonate concentration. Adrenergic stimulation did not affect the intracellular pH.

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Documento generato il 02/04/20 alle ore 12:21:58