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Titolo:
Cotransplantation of stroma results in enhancement of engraftment and early expression of donor hematopoietic stem cells in utero
Autore:
Almeida-Porada, G; Flake, AW; Glimp, HA; Zanjani, ED;
Indirizzi:
Univ Nevada, Dept Vet Affairs Med Ctr, Dept Med, Reno, NV 89520 USA Univ Nevada Reno NV USA 89520 fairs Med Ctr, Dept Med, Reno, NV 89520 USA Univ Nevada, Dept Vet Affairs Med Ctr, Dept Vet Med, Reno, NV 89520 USA Univ Nevada Reno NV USA 89520 s Med Ctr, Dept Vet Med, Reno, NV 89520 USA Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 10, volume: 27, anno: 1999,
pagine: 1569 - 1575
SICI:
0301-472X(199910)27:10<1569:COSRIE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; BONE-MARROW STROMA; TRANSPLANTATION; LIVER; MICE; MICROENVIRONMENT; INUTERO; CHIMERISM; PROGENITORS; MECHANISMS;
Keywords:
hematopoietic stem cells; stroma; in utero transplantation; engraftment; sheep;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Almeida-Porada, G Univ Nevada, Dept Vet Affairs Med Ctr, Dept Med, 1000 Locust St 151B, Reno, NV 89520 USA Univ Nevada 1000 Locust St 151B Reno NV USA 89520 20 USA
Citazione:
G. Almeida-Porada et al., "Cotransplantation of stroma results in enhancement of engraftment and early expression of donor hematopoietic stem cells in utero", EXP HEMATOL, 27(10), 1999, pp. 1569-1575

Abstract

Although promising, clinical and experimental efforts at in utero hematopoietic stem cell (HSC) transplantation currently are limited by minimal donor cell engraftment and lack of early donor cell expression after transplantation. We reasoned that cotransplantation of stromal elements (ST) might condition the fetal microenvironment for the engraftment of donor HSC and facilitate precocious bone marrow (BR I) hematopoiesis. In this study we cotransplanted sheep ST, derived from adult or fetal BR I, with either adult or fetal HSC, into preimmune fetal sheep, We analyzed donor cell chimerism in BM and peripheral blood and compared levels of chimerism achieved with recipients of NSC alone. In all experimental groups, stromal cotransplantation markedly increased the level of peripheral blood donor cell expression at 60 days after transplantation relative to controls. Adult BM-derived stroma cotransplanted with adult HSC provided the highest levels of circulating donor cells, whereas fetal-derived stroma was less effective, In addition, STcotransplantation resulted in increased donor cell engraftment in the BM and led to significantly increased levels of donor hematopoiesis for over 30months after transplant, Cotransplantation of stroma may represent a valuable clinical strategy for optimal application of in utero HSC transplantation. (C) 1999 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 27/11/20 alle ore 22:10:28